EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Asymmetric sheath coordination controls flagellar architecture and function in Leptospira spirochete.
ジャーナル・号・ページ	Embo J., Vol. 45, Page 2882-2904, Year 2026
掲載日	2025年10月18日
著者	Akihiro Kawamoto / Toshiki Kuribayashi / Masatomo Morita / Shuichi Nakamura / Nobuo Koizumi /
PubMed 要旨	Bacterial flagella are essential for motility, but their structure and how they generate movement vary greatly. Most motile bacteria use external helical flagella, whereas spirochetes have ...Bacterial flagella are essential for motility, but their structure and how they generate movement vary greatly. Most motile bacteria use external helical flagella, whereas spirochetes have periplasmic flagella (PFs) that distort the cell body to drive forward movement. Here, we generated sheath protein knockout mutants and used high-resolution cryo-electron microscopy to elucidate the mechanisms underlying PF assembly, curvature, and rigidity in Leptospira biflexa. The PF consists of a FlaB1-based core filament surrounded asymmetrically by sheath proteins. Weak but essential binding of FlaA2 to the core enables asymmetric localization of the coiling protein FcpA. FcpA alone can induce curvature, whereas FcpB acts as a structural wedge that reinforces PF rigidity and enables efficient swimming in liquid. Specific glycosylation of FlaB1 mediates sheath-core interactions and may guide the assembly of sheath components. We propose that sheath proteins interact transiently with the core and may be anchored to the outer membrane, allowing core rotation beneath a static sheath. These findings reveal how cooperative interactions among sheath components confer structural and mechanical specialization to spirochete flagella.
リンク	Embo J. / PubMed:41844841 / PubMed Central
手法	EM (らせん対称) / EM (単粒子)
解像度	2.3 - 4.35 Å
構造データ	63347 9lry EMDB-63347, PDB-9lry: Straight and symmetrical filament of the spirochete periplasmic flagella of Leptospira biflexa 手法: EM (らせん対称) / 解像度: 2.3 Å 63348 9lrz EMDB-63348, PDB-9lrz: Core filament of the spirochete periplasmic flagella of Leptospira biflexa 手法: EM (らせん対称) / 解像度: 2.4 Å 63349 9ls0 EMDB-63349, PDB-9ls0: Straight and symmetrical filament of the spirochete periplasmic flagella of Leptospira biflexa deleted fcpB strain 手法: EM (らせん対称) / 解像度: 2.4 Å 63350 9ls1 EMDB-63350, PDB-9ls1: core filament of the spirochete periplasmic flagella of Leptospira biflexa deleted fcpB strain 手法: EM (らせん対称) / 解像度: 2.4 Å 66641 9x7k EMDB-66641, PDB-9x7k: core filament of the spirochete periplasmic flagella of Leptospira biflexa wild type 手法: EM (単粒子) / 解像度: 4.35 Å 66642 9x7l EMDB-66642, PDB-9x7l: core filament of the spirochete periplasmic flagella of Leptospira biflexa from the flaA2-complemented stain 手法: EM (単粒子) / 解像度: 3.32 Å 66643 9x7m EMDB-66643, PDB-9x7m: core filament of the spirochete periplasmic flagella of Leptospira biflexa from the deleted fcpB_CL13 strain 手法: EM (単粒子) / 解像度: 3.2 Å 66646 9x7s EMDB-66646, PDB-9x7s: sheathed filament of the spirochete periplasmic flagella of Leptospira biflexa from the flaA2-complemented stain 手法: EM (単粒子) / 解像度: 2.83 Å 66647 9x7v EMDB-66647, PDB-9x7v: Sheathed filament of the spirochete periplasmic flagella of Leptospira biflexa from the deleted fcpB_CL13 strain 手法: EM (単粒子) / 解像度: 2.7 Å 66649 9x80 EMDB-66649, PDB-9x80: sheathed filament of the spirochete periplasmic flagella of Leptospira biflexa wild type 手法: EM (単粒子) / 解像度: 3.24 Å
由来	leptospira biflexa (バクテリア)
キーワード	MOTOR PROTEIN / Filament / Flagellar motor