EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural mechanism of SAM-AMP and SAM-AMP synthesis by the type III-D2 CRISPR effector complex.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Year 2026
掲載日	2026年6月24日
著者	Yoshihisa Mitsuda / Maki Sugaya / Junichiro Ishikawa / Naoto Nagahata / Sae Okazaki / Masahiro Hiraizumi / Kazuki Kato / Jonathan S Gootenberg / Omar O Abudayyeh / Tsuyoshi Osawa / Keitaro Yamashita / Hiroshi Nishimasu /
PubMed 要旨	The type III-D2 CRISPR-Cas system comprises multiple Cas subunits and a CRISPR RNA, and is likely an evolutionary intermediate between the well-studied type III-A and III-E systems. Here we show that ...The type III-D2 CRISPR-Cas system comprises multiple Cas subunits and a CRISPR RNA, and is likely an evolutionary intermediate between the well-studied type III-A and III-E systems. Here we show that the type III-D2 complex synthesizes two distinct second messengers, SAM-AMP and SAM-AMP, from S-adenosylmethionine (SAM) and ATP in response to target RNA recognition. We determined cryo-electron microscopy structures of the type III-D2 effector complex in different functional states, providing mechanistic insights into target RNA cleavage and second messenger synthesis. The structures reveal how SAM and ATP are recognized by the Cas10 subunit within the effector complex. Furthermore, our biological data suggest that both SAM-AMP and SAM-AMP act on the CorA ancillary effector, inducing growth arrest of infected bacterial cells and thereby conferring immunity. Thus, our study establishes the type III-D2 system as a unique anti-phage defense mechanism that employs both SAM-AMP and SAM-AMP as second messengers, expanding the repertoire of second messenger strategies in bacterial defense systems and highlighting the remarkable functional diversity of CRISPR-Cas systems.
リンク	Nat Commun / PubMed:42342668 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 2.9 Å
構造データ	66346 9wxh EMDB-66346, PDB-9wxh: Cryo-EM structure of the type III-D2 CRISPR-Cas effector complex bound to a cognate target RNA in the pre-cleavage state 手法: EM (単粒子) / 解像度: 2.6 Å 66347 9wxi EMDB-66347, PDB-9wxi: Cryo-EM structure of the type III-D2 CRISPR-Cas effector complex bound to a cognate target RNA in the post-cleavage state 手法: EM (単粒子) / 解像度: 2.8 Å 66348 9wxj EMDB-66348, PDB-9wxj: Cryo-EM structure of the type III-D2 CRISPR-Cas effector complex bound to a non-cognate target RNA in the pre-cleavage state 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-69899: Cryo-EM structure of the type III-D2 CRISPR-Cas effector complex bound to a cognate target RNA in the pre-cleavage state 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-69900: Cryo-EM structure of the type III-D2 CRISPR-Cas effector complex bound to a cognate target RNA in the post-cleavage state 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-69901: Cryo-EM structure of the type III-D2 CRISPR-Cas effector complex bound to a non-cognate target RNA in the pre-cleavage state 手法: EM (単粒子) / 解像度: 2.8 Å
化合物	ChemComp-MG: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-SAM: S-ADENOSYLMETHIONINE ChemComp-ZN: Unknown entry ChemComp-HOH*: WATER
由来	gammaproteobacteria bacterium (バクテリア)
キーワード	IMMUNE SYSTEM / CRISPR / Anti-phage defense / Second messenger / SAM-AMP