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Structure paper

TitleStructural basis of AtCas9 recognition of PAM mutants in underwound DNA topology
Journal, issue, pagesNat. Struct. Mol. Biol., Year 2026
Publish dateDec 9, 2025 (structure data deposition date)
AuthorsDuan, M. / Meng, B. / Zhou, L. / Wu, L.J. / Tong, X. / Huang, D. / Yin, H. / Liu, Z.J. / Zhang, Y.
External linksNat. Struct. Mol. Biol. / Search PubMed
MethodsEM (single particle)
Resolution2.41 - 3.15 Å
Structure data

PDB-21dz:
Cryo-EM structure of AtCas9-sgRNA-underwound DNA (CATA PAM) ternary complex
Method: ELECTRON MICROSCOPY / Resolution: 2.41 Å

PDB-21ea:
Cryo-EM structure of AtCas9-sgRNA-underwound DNA (TATA PAM) ternary complex
Method: ELECTRON MICROSCOPY / Resolution: 2.65 Å

PDB-9wac:
Cryo-EM structure of AtCas9-sgRNA-underwound DNA (TTGA) ternary complex
Method: ELECTRON MICROSCOPY / Resolution: 2.89 Å

PDB-9wad:
Cryo-EM structure of AtCas9-sgRNA-B-form DNA ternary complex
Method: ELECTRON MICROSCOPY / Resolution: 3.15 Å

Source
  • alicyclobacillus tolerans (bacteria)
KeywordsRNA BINDING PROTEIN/RNA/DNA / Complex / Endonuclease / Immunity / RNA BINDING PROTEIN-RNA-DNA complex

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