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TitleCoupling of polymerase-nucleoprotein-RNA in an influenza virus mini ribonucleoprotein complex.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 9741, Year 2025
Publish dateNov 4, 2025
AuthorsHuiling Kang / Yunxiang Yang / Yixiao Liu / Mingyu Li / Lejin Zhang / Yuqi Lin / Leander Witte / Kuang-Yu Chen / Wenya Song / Zhili Xu / Xiaojing He / Luke W Guddat / Yu Guo / Liming Yan / Yan Gao / Ervin Fodor / Zihe Rao / Zhiyong Lou /
PubMed AbstractInfluenza virus ribonucleoprotein complexes (RNPs), composed of the polymerase complex (FluPol), nucleoprotein (NP), and RNA, are essential for replication and transcription. We report atomic- ...Influenza virus ribonucleoprotein complexes (RNPs), composed of the polymerase complex (FluPol), nucleoprotein (NP), and RNA, are essential for replication and transcription. We report atomic-resolution cryo-EM structures of mini-vRNPs in two states: FluPol located inside (State-In) or at the outer rim (State-Out) of the NP-RNA ring. In both states, the 5' and 3' termini of vRNA are bound to FluPol as previously reported. One NP (NP-0) contacts PA/PB1 of FluPol and binds the distal double-stranded vRNA promoter, with its D72-K90 loop inserting into the RNA fork; separated strands occupy NP-0 RNA-binding grooves. Grooves from other NPs form a continuous RNA-protective path, consistent with negative-strand RNA virus mechanisms. In State-In, interfaces for FluPol dimerization or Pol II interaction are blocked, but fully exposed in State-Out. These structures reveal detailed FluPol-NP-RNA coupling and suggest a conformational shift in RNPs during the viral life cycle.
External linksNat Commun / PubMed:41188214 / PubMed Central
MethodsEM (single particle)
Resolution2.97 - 4.75 Å
Structure data

64766

9v44

EMDB-64766, PDB-9v44:
Coupling of polymerase-nucleoprotein-RNA in an influenza virus mini ribonucleoprotein complex
Method: EM (single particle) / Resolution: 2.97 Å

64768

9v46

EMDB-64768, PDB-9v46:
Coupling of polymerase-nucleoprotein-RNA in an influenza virus mini ribonucleoprotein complex
Method: EM (single particle) / Resolution: 3.62 Å

64769

9v47

EMDB-64769, PDB-9v47:
Coupling of polymerase-nucleoprotein-RNA in an influenza virus mini ribonucleoprotein complex
Method: EM (single particle) / Resolution: 4.52 Å

64770

9v48

EMDB-64770, PDB-9v48:
Coupling of polymerase-nucleoprotein-RNA in an influenza virus mini ribonucleoprotein complex
Method: EM (single particle) / Resolution: 4.75 Å

Source
  • influenza a virus (a/victoria/3/1975(h3n2))
KeywordsVIRAL PROTEIN/RNA / Influenza virus / ribonucleoprotein complex / polymerase / cryo-EM / VIRAL PROTEIN-RNA complex

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