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Structure paper

TitleStructure mechanism of the avian R2 retrotransposon for programmable gene integration
Journal, issue, pagesTo Be Published
Publish dateApr 6, 2025 (structure data deposition date)
AuthorsKomatsu D / Nishimasu H
External linksSearch PubMed
MethodsEM (single particle)
Resolution2.19 - 3.3 Å
Structure data

64054

9ucx

EMDB-64054, PDB-9ucx:
Cryo-EM structure of R2Tg bound to template RNA and target DNA in the target-primed reverse transcription initiation state (state 1)
Method: EM (single particle) / Resolution: 2.19 Å

64055

9ucy

EMDB-64055: Cryo-EM structure of R2Tg bound to template RNA and target DNA in the target-primed reverse transcription initiation state (state 2)
PDB-9ucy: Cryo-EM structure of the R2Tg retrotransposon in complex with R2RNA and rDNA in the initiating target-primed reverse transcription state (top strand cleaved)
Method: EM (single particle) / Resolution: 2.54 Å

64056

9ucz

EMDB-64056: Cryo-EM structure of R2Tg bound to template RNA and target DNA in the target-primed reverse transcription initiation state (state 3)
PDB-9ucz: Cryo-EM structure of the R2Tg retrotransposon in complex with R2RNA and rDNA in the initiating target-primed reverse transcription state (ZF3/2 disordered)
Method: EM (single particle) / Resolution: 2.61 Å

64057

9ud0

EMDB-64057, PDB-9ud0:
Cryo-EM structure of R2Tg bound to template RNA and target DNA in the target-primed reverse transcription initiation state (state 1, Myb domain focused)
Method: EM (single particle) / Resolution: 3.14 Å

64058

9ud1

EMDB-64058, PDB-9ud1:
Cryo-EM structure of STITCHR (R2Tg and Cas9 nickase) bound to guide RNA, template RNA, and target DNA in the target-primed reverse transcription initiation state
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-TTP:
THYMIDINE-5'-TRIPHOSPHATE

ChemComp-HOH:
WATER

Source
  • taeniopygia guttata (zebra finch)
  • streptococcus pyogenes (bacteria)
  • cloning vector pet-j23100-laco(symr+1)-gfp-lva (others)
KeywordsDNA BINDING PROTEIN / retrotransposon / LINE / reverse transcriptase / endonuclease / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA-DNA complex

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