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TitleCryoEM structural analysis of a thermophilic galactooligosaccharides-producer β-galactosidase unravels an uncommon oligomeric structure.
Journal, issue, pagesInt J Biol Macromol, Vol. 362, Page 151980, Year 2026
Publish dateApr 13, 2026
AuthorsGennaro Sanità / Emanuela Maresca / Stefano Capaldi / Angela Casillo / Martina Aulitto / Federica Donadio / Tillmann Pape / Maria Michela Corsaro / Emanuela Esposito / Patrizia Contursi /
PubMed AbstractThermostable β-galactosidases represent promising biocatalysts for lactose hydrolysis and production of structurally defined galacto-oligosaccharides (GOS). Here we report the cryo-EM structure of ...Thermostable β-galactosidases represent promising biocatalysts for lactose hydrolysis and production of structurally defined galacto-oligosaccharides (GOS). Here we report the cryo-EM structure of the glycoside hydrolase family 42 (GH42) β-galactosidase from Heyndrickxia coagulans MA-13 (HcGalB), determined at 2.97 Å resolution. HcGalB adopts a canonical tripartite architecture and assembles into a barrel-like homo-hexamer composed of two staggered trimers that interact in an unusual top-to-top configuration. This quaternary arrangement contributes not only to structural stability but also to the modulation of substrate channeling and catalytic properties. Molecular docking revealed a surface groove shaped by conserved aromatic residues that might guide the substrate towards the catalytic pocket. Moreover, the structural data provide a mechanistic rationale for the efficient transgalactosylation activity of HcGalB, which predominantly generates β (1 → 3)-linked GOS, along with β(1 → 6) and β(1 → 4) linkages, as confirmed by 2D Nuclear Magnetic Resonance. Overall, these findings expand the structural landscape of GH42 enzymes and identify architecture-specific determinants that can be leveraged to optimize GH42 catalysts for industrial and functional food applications.
External linksInt J Biol Macromol / PubMed:41985809
MethodsEM (single particle)
Resolution2.97 Å
Structure data

55743

9ta3

EMDB-55743, PDB-9ta3:
Cryo-EM structure of Heyndrickxia coagulans beta-galactosidase
Method: EM (single particle) / Resolution: 2.97 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • heyndrickxia coagulans (bacteria)
KeywordsHYDROLASE / beta-galactosidase / Heyndrickxia coagulans / transgalactosylation

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