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TitleOpen and closed forms of assembled henipavirus nucleoprotein suggest structural basis of genome access.
Journal, issue, pagesSci Adv, Vol. 12, Issue 20, Page eaed8300, Year 2026
Publish dateMay 15, 2026
AuthorsRupesh Balaji Jayachandran / Erwan Quignon / Max Renner /
PubMed AbstractHenipaviruses, such as Nipah virus, can cause deadly illness and constitute WHO blueprint priorities due to their pandemic potential. Their genomes are packaged within a nucleocapsid consisting of ...Henipaviruses, such as Nipah virus, can cause deadly illness and constitute WHO blueprint priorities due to their pandemic potential. Their genomes are packaged within a nucleocapsid consisting of viral nucleoproteins (N). Now, it is unclear how the encapsidated genome is released from N to allow the viral polymerase to read its sequence. Here, we present the high-resolution cryo-EM structure of a helical N-RNA filament from Langya henipavirus (LayV), allowing us to identify vertical interactions crucial for assembly. We show that assembly efficiency is sequence-dependent and prefers 5'-genomic sequences. Further, we solve the structure of an RNA-free assembly of LayV-N. Structural comparison of the RNA-bound and RNA-free LayV-N shows a conformational opening and closing, even within the assembled state. Our data suggest that N within nucleocapsids may undergo local conformational changes, switching between closed and open states, to temporarily allow access to the encapsidated RNA without nucleocapsid disruption.
External linksSci Adv / PubMed:42127178 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution2.6 - 3.1 Å
Structure data

55586

9t5k

EMDB-55586, PDB-9t5k:
Local refinement of RNA-free assembled Langya virus N-core
Method: EM (single particle) / Resolution: 2.6 Å

55587

9t5l

EMDB-55587, PDB-9t5l:
Helical reconstruction of Langya henipavirus N-core nucleocapsid-like complex
Method: EM (helical sym.) / Resolution: 3.1 Å

EMDB-55588: Langya henipavirus Ncore 13mer Ring
Method: EM (single particle) / Resolution: 2.6 Å

Source
  • langya virus
  • synthetic construct (others)
KeywordsVIRAL PROTEIN / Henipavirus / Langya virus / cryo-EM / nucleocapsid / nucleoprotein / RNA

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