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TitleCatalytic Cycle of N-Acetylmuramic Acid-alpha-1-Phosphate Uridylyltransferase MurU of Pseudomonas aeruginosa
Journal, issue, pagesAcs Catalysis, Year 2026
Publish dateSep 9, 2025 (structure data deposition date)
AuthorsJimenez-Faraco, E. / El-Araby, A.M. / Feltzer, R. / Nguyen, V.T. / Mobashery, S. / Hermoso, J.A.
External linksAcs Catalysis / Search PubMed
MethodsX-ray diffraction
Resolution1.6 - 2.2 Å
Structure data

PDB-9smv:
N-acetylmuramate alpha-1-phosphate uridylyltransferase (MurU) in complex with UTP/Mg
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-9sqd:
PaMurU in complex with their natural substrates (UTP and NAM-1P) and Mg2+ cofactor
Method: X-RAY DIFFRACTION / Resolution: 1.951 Å

PDB-9sqe:
PaMurU in complex with Mg2+ cofactor and UDPNAM/Diphosphate products
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-9sqf:
PaMurU in complex with Mn2+ and UTP substrate
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-9sqg:
PaMurU in complex with Mn2+ and UDPNAM (uridine diphosphate N-acetyl muramic acid)
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-9szk:
PaMurU in complex with Ca2+ and UTP substrate
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-9szl:
PaMurU in complex with Ca2+ and UDPNAM (uridine diphosphate N-acetyl muramic acid)
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

Chemicals

ChemComp-UTP:
URIDINE 5'-TRIPHOSPHATE / UTP*YM

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-491:
2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-alpha-D-glucopyranose

ChemComp-DPO:
DIPHOSPHATE

ChemComp-EPZ:
(2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-MN:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-PO4:
PHOSPHATE ION

ChemComp-CA:
Unknown entry

ChemComp-PGE:
TRIETHYLENE GLYCOL

Source
  • pseudomonas aeruginosa (bacteria)
KeywordsTRANSFERASE / Pseudonomas aeruginosa Peptidoglycan recycling pathway Bacteria cell wall

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