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TitleStructural Basis of Lipopolysaccharide O-Antigen Chain Length Modality.
Journal, issue, pagesResearch (Wash D C), Vol. 9, Page 1276, Year 2026
Publish dateMay 12, 2026
AuthorsBenjamin Wiseman / Göran Widmalm / Martin Högbom /
PubMed AbstractLipopolysaccharides are important components of the gram-negative bacterial cell envelope that are involved in immune evasion and act as a protective barrier. Employing cryo-electron microscopy, we ...Lipopolysaccharides are important components of the gram-negative bacterial cell envelope that are involved in immune evasion and act as a protective barrier. Employing cryo-electron microscopy, we resolved the structure and dynamics of FepE, the copolymerase component of the Wzy-dependent pathway, responsible for the length modulation of very long O-antigen molecules. Comparison of the interior volumes of related copolymerases' periplasmic domains with the volume of hydrated sugars suggests that the size of the periplasmic domain controls the length of the O-antigen, implying that polysaccharide chain polymerization occurs inside the copolymerase periplasmic domain. Moreover, we show the opening of the FepE complex as well as other large mechanistically relevant movements. The opening of the complex presents an attractive corridor for the release of completed polysaccharide chains.
External linksResearch (Wash D C) / PubMed:42131584 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 3.4 Å
Structure data

54965

9ski

EMDB-54965, PDB-9ski:
Polysaccharide co-polymerase FepE open 7 subunit complex
Method: EM (single particle) / Resolution: 3.1 Å

54966

9skj

EMDB-54966, PDB-9skj:
Polysaccharide co-polymerase FepE open 8 subunit complex
Method: EM (single particle) / Resolution: 3.1 Å

54967

9skk

EMDB-54967, PDB-9skk:
Polysaccharide co-polymerase FepE open 9 subunit complex
Method: EM (single particle) / Resolution: 3.3 Å

54968

9skl

EMDB-54968, PDB-9skl:
Polysaccharide co-polymerase FepE closed 9 subunit complex C9 symmetry
Method: EM (single particle) / Resolution: 2.8 Å

EMDB-54969: Polysaccharide co-polymerase closed FepE 9 subunit complex C1 symmetry.
Method: EM (single particle) / Resolution: 3.4 Å

Source
  • escherichia coli k-12 (bacteria)
KeywordsMEMBRANE PROTEIN / enzyme / polysaccharide / co-polymerase

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