EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular mechanism of co-transcriptional H3K36 methylation by SETD2.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 9565, Year 2025
掲載日	2025年10月29日
著者	James L Walshe / Moritz Ochmann / Ute Neef / Olexandr Dybkov / Christian Dienemann / Christiane Oberthür / Aiturgan Zheenbekova / Henning Urlaub / Patrick Cramer /
PubMed 要旨	H3K36me3 is a hallmark of actively and recently transcribed genes and contributes to cellular memory and identity. The deposition of H3K36me3 occurs co-transcriptionally when the methyltransferase ...H3K36me3 is a hallmark of actively and recently transcribed genes and contributes to cellular memory and identity. The deposition of H3K36me3 occurs co-transcriptionally when the methyltransferase SETD2 associates with RNA polymerase II. Here we present three cryo-EM structures of SETD2 bound to RNA polymerase II elongation complexes at different states of nucleosome passage. Together with functional probing, our results suggest a 3-step mechanism of transcription-coupled H3K36me3 deposition. First, binding to the elongation factor SPT6 tethers the catalytic SET domain in proximity to the upstream DNA. Second, RNA polymerase II nucleosome passage leads to the transfer of a hexasome from downstream to upstream, poised for methylation. Finally, continued transcription leads to upstream nucleosome reassembly, partial dissociation of the histone chaperone FACT and sequential methylation of both H3 tails, completing H3K36me3 deposition of an upstream nucleosome after RNA polymerase II passage.
リンク	Nat Commun / PubMed:41162378 / PubMed Central
手法	EM (単粒子)
解像度	3.58 - 6.72 Å
構造データ	51643 9gw2 EMDB-51643, PDB-9gw2: State 2 MAP 1 SETD2 bound to proximal H3 of upstream nucleosome 手法: EM (単粒子) / 解像度: 4.84 Å 54247 9rtn EMDB-54247, PDB-9rtn: State 2 MAP 2 SPT6 with SETD2 手法: EM (単粒子) / 解像度: 3.82 Å 54399 9rzc EMDB-54399, PDB-9rzc: State 3 MAP 1 SETD2 bound to distal H3 of upstream nucleosome 手法: EM (単粒子) / 解像度: 3.66 Å 54400 9rzd EMDB-54400, PDB-9rzd: State 3 MAP 2 SPT6 with SETD2 手法: EM (単粒子) / 解像度: 3.69 Å 54425 9s0u EMDB-54425, PDB-9s0u: State 3 MAP 3 RNA Pol II activated elongation complex with SETD2 bound to distal upstream H3 手法: EM (単粒子) / 解像度: 6.72 Å 54537 9s3g EMDB-54537, PDB-9s3g: State 1 MAP3 RNA Pol II activated elongation complex with SETD2 and upstream hexasome 手法: EM (単粒子) / 解像度: 6.4 Å EMDB-54538: State 1 MAP1 RNA Pol II activated elongation complex with SETD2 and upstream hexasome 手法: EM (単粒子) / 解像度: 3.89 Å EMDB-54541: State 1 MAP2 RNA Pol II activated elongation complex with SETD2 and upstream hexasome 手法: EM (単粒子) / 解像度: 3.71 Å EMDB-54542: State 1 MAP4 RNA Pol II activated elongation complex with SETD2 and upstream hexasome 手法: EM (単粒子) / 解像度: 3.58 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry
由来	homo sapiens (ヒト) synthetic construct (人工物) sus scrofa (ブタ)
キーワード	TRANSCRIPTION / RNA Pol II Activated elongation complex Co-transcriptional H3K36me3 SETD2