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Structure paper

TitleDynamic oligomerisation states of replicative helicase 40 coordinate during adeno-associated virus packaging
Journal, issue, pagesTo Be Published
Publish dateJun 17, 2025 (structure data deposition date)
AuthorsXu V / Barritt JD / Bubeck D / Wake M / Rouse SL
External linksSearch PubMed
MethodsEM (single particle)
Resolution1.88 - 3.08 Å
Structure data

EMDB-54050, PDB-9rm5:
Hexameric AAV2 Rep40-ssDNA (ITR) complex in presence of ATPyS
Method: EM (single particle) / Resolution: 2.44 Å

EMDB-54184, PDB-9rqt:
Heptameric AAV2 Rep40-dsDNA (ITR) complex in presence of ATPgS
Method: EM (single particle) / Resolution: 2.88 Å

EMDB-54195, PDB-9rrs:
AAV8 capsid in complex with Rep40 and ADP
Method: EM (single particle) / Resolution: 1.88 Å

EMDB-54328, PDB-9rwg:
Pentameric AAV2 Rep40 in complex with AAV8
Method: EM (single particle) / Resolution: 2.19 Å

EMDB-54416, PDB-9s0n:
Hexameric AAV2 Rep40-dsDNA (ITR) melting complex in presence of ATPyS
Method: EM (single particle) / Resolution: 3.08 Å

EMDB-54429, PDB-9s10:
Hexameric AAV2 Rep40-dsDNA (ITR) duplex complex in presence of ATPyS
Method: EM (single particle) / Resolution: 3.07 Å

Chemicals

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-HOH:
WATER

Source
  • adeno-associated virus 2
  • dna molecule (others)
  • adeno-associated virus 8
KeywordsDNA BINDING PROTEIN / Helicase / Adeno-associated Virus / AAA+ ATPase / VIRUS / AAV

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