EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural and functional mapping of protective human monoclonal antibodies against enterovirus A71.
ジャーナル・号・ページ	Sci Adv, Vol. 12, Issue 23, Page eaee8217, Year 2026
掲載日	2026年6月5日
著者	Daming Zhou / Abhay Kotecha / James T Kelly / Peng-Nien Huang / Yi-Yin Chen / Thomas S Walter / Helen M E Duyvesteyn / Raymond J Owens / Shu-Yuan Ho / Tzou-Yien Lin / Elizabeth E Fry / Jingshan Ren / Kuan-Ying A Huang / David I Stuart /
PubMed 要旨	EV-A71 has been responsible for recent severe HFMD outbreaks. We report structures for 12 potently neutralizing human anti-EV-A71 monoclonal antibody Fabs, alone and complexed with virus. Most ...EV-A71 has been responsible for recent severe HFMD outbreaks. We report structures for 12 potently neutralizing human anti-EV-A71 monoclonal antibody Fabs, alone and complexed with virus. Most recognize the native antigenic state with epitopes that span interfaces, together covering 85% of the capsid surface. The majority (8 of 12) bind the canyon, while the others cluster around the icosahedral two- and threefold axes. Blocking SCARB2 receptor binding likely contributes to neutralization for all, and a subset induces empty particles. A predominant gene family (IGHV4-39) does not dictate a common binding pose. Long CDR-H3 loops are frequently key to binding, especially at the canyon, suggesting that antigenicity data based on antibodies with shorter CDR3s (e.g., murine) may be misleading. This dataset reveals neutralization mechanisms for recently circulating EV-A71 genotypes, which will inform immunotherapies. We demonstrate synergy in vitro between canyon binding and both two- and threefold binding antibodies to increase neutralization potency.
リンク	Sci Adv / PubMed:42247493 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.14 - 4.8 Å
構造データ	53994 9rig EMDB-53994, PDB-9rig: EV-A71 (genotype B2) in complex with 16-2-9D Fab 手法: EM (単粒子) / 解像度: 3.7 Å 53995 9rih EMDB-53995, PDB-9rih: EV-A71 (genotype B2) in complex with 16-2-12D Fab 手法: EM (単粒子) / 解像度: 3.1 Å 53996 9rii EMDB-53996, PDB-9rii: EV-A71 (genotype B2) in complex with 34-1-6D Fab 手法: EM (単粒子) / 解像度: 3.0 Å 53997 9rij EMDB-53997, PDB-9rij: EV-A71 (genotype B5) in complex with 16-2-8C Fab 手法: EM (単粒子) / 解像度: 3.8 Å 53998 9rik EMDB-53998, PDB-9rik: EV-A71 (genotype B5) in complex with 16-2-2D Fab 手法: EM (単粒子) / 解像度: 3.4 Å 53999 9ril EMDB-53999, PDB-9ril: EV-A71 (genotype B5) in complex with 16-3-3C Fab 手法: EM (単粒子) / 解像度: 3.6 Å 54000 9rim EMDB-54000, PDB-9rim: EV-A71 (genotype B5) in complex with 16-3-4D Fab 手法: EM (単粒子) / 解像度: 3.0 Å 54001 9rin EMDB-54001, PDB-9rin: EV-A71 (genotype B5) in complex with 17-1-12A Fab 手法: EM (単粒子) / 解像度: 4.1 Å 54002 9rio EMDB-54002, PDB-9rio: EV-A71 (genotype B5) in complex with 17-2-2B Fab 手法: EM (単粒子) / 解像度: 3.4 Å 54003 9rip EMDB-54003, PDB-9rip: EV-A71 (genotype B5) in complex with 17-2-12A Fab 手法: EM (単粒子) / 解像度: 3.1 Å 54004 9riq EMDB-54004, PDB-9riq: EV-A71 (genotype C4) in complex with 16-2-11B Fab 手法: EM (単粒子) / 解像度: 3.3 Å 54005 9rir EMDB-54005, PDB-9rir: EV-A71 (genotype C4) in complex with 16-3-10B Fab 手法: EM (単粒子) / 解像度: 3.3 Å 55620 9t6z EMDB-55620, PDB-9t6z: EV-A71 (genotype B5) empty particle in complex with 16-3-3C Fab 手法: EM (単粒子) / 解像度: 4.8 Å PDB-9i3w: Structure of anti-EV71 human monoclonal antibody 16-2-8C Fab 手法: X-RAY DIFFRACTION / 解像度: 1.14 Å PDB-9i3x: Structure of anti-EV71 human monoclonal antibody 16-2-11B Fab 手法: X-RAY DIFFRACTION / 解像度: 2.57 Å PDB-9i3z: Structure of anti-EV71 human monoclonal antibody 16-2-12D Fab 手法: X-RAY DIFFRACTION / 解像度: 3.06 Å PDB-9i40: Structure of anti-EV71 human monoclonal antibody 16-3-4D Fab 手法: X-RAY DIFFRACTION / 解像度: 2.78 Å PDB-9i41: Structure of anti-EV71 human monoclonal antibody 16-3-3C Fab 手法: X-RAY DIFFRACTION / 解像度: 1.32 Å PDB-9i42: Structure of anti-EV71 human monoclonal antibody 16-2-2D Fab 手法: X-RAY DIFFRACTION / 解像度: 1.92 Å PDB-9i43: Structure of anti-EV71 human monoclonal antibody 16-2-9D Fab 手法: X-RAY DIFFRACTION / 解像度: 1.18 Å PDB-9i45: Structure of anti-EV71 human monoclonal antibody 16-3-10B Fab 手法: X-RAY DIFFRACTION / 解像度: 2.12 Å PDB-9i4b: Structure of anti-EV71 human monoclonal antibody 17-2-12A Fab 手法: X-RAY DIFFRACTION / 解像度: 2.68 Å PDB-9i4c: Structure of anti-EV71 human monoclonal antibody 17-1-12A Fab 手法: X-RAY DIFFRACTION / 解像度: 2.83 Å PDB-9i4d: Structure of anti-EV71 human monoclonal antibody 17-2-2B Fab 手法: X-RAY DIFFRACTION / 解像度: 2.56 Å PDB-9i4e: Structure of anti-EV71 human monoclonal antibody 34-1-6D Fab 手法: X-RAY DIFFRACTION / 解像度: 1.63 Å
化合物	ChemComp-BTB: 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH緩衝剤YM ChemComp-EDO: 1,2-ETHANEDIOL ChemComp-HOH: WATER ChemComp-FMT: FORMIC ACID ChemComp-IMD: IMIDAZOLE ChemComp-SO4: SULFATE ION ChemComp-GOL: GLYCEROL ChemComp-CL: Unknown entry ChemComp-ACT: ACETATE ION ChemComp-SPH*: SPHINGOSINE
由来	Enterovirus A71 (エンテロウイルス) homo sapiens (ヒト) chlorocebus aethiops (ミドリザル) human enterovirus 71 (エンテロウイルス)
キーワード	ANTIVIRAL PROTEIN / EV71 / antibody / 16-2-8C / Fab / 16-2-11B / 16-2-12D / 16-3-4D / 16-3-3C / 16-2-2D / 16-2-9D / 16-3-10B / 17-2-12A / 17-1-12A / 17-2-2B / 34-1-6D / VIRUS / Hand-foot-and-mouth disease / HFMD / enterovirus 71 (EV-A71) / Human antibody / 17-1-2B