Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure of a contractile injection system in Salmonella enterica subsp. salamae.
Journal, issue, pages	Nat Commun, Year 2026
Publish date	Apr 16, 2026
Authors	Rooshanie N Ejaz / Kristin Funke / Claudia S Kielkopf / Freddie J O Martin / Marta Šiborová / Ivo A Hendriks / Nicholas H Sofos / Tillmann Pape / Eva M Steiner-Rebrova / Michael L Nielsen / Marc Erhardt / Nicholas M I Taylor /
PubMed Abstract	Extracellular contractile injection systems (eCISs) are phage-derived nanomachines used by bacteria to deliver effectors into target cells. Well-studied examples include the Photorhabdus asymbiotica ...Extracellular contractile injection systems (eCISs) are phage-derived nanomachines used by bacteria to deliver effectors into target cells. Well-studied examples include the Photorhabdus asymbiotica virulence cassettes and the antifeeding prophage from Serratia entomophila, which have been engineered for heterologous cargo delivery. Recent genomic analyses identified eCIS gene clusters in the opportunistic human pathogen Salmonella enterica subspecies salamae, but their structure, function, and biotechnological potential remain unexplored. Here, we report a high-resolution cryo-electron microscopy structure of the S. enterica eCIS. Our atomic models reveal a distinctive sheath architecture, an expansive cage-like shell around a central spike, and an associated integral membrane protein. We identify a putative effector encoded within the operon exhibiting mild periplasmic toxicity and provide evidence that the S. enterica eCIS deviates from canonical eCISs by interacting with the inner membrane. Guided by these structural features, we uncover, to the best of our knowledge, a previously unannotated cluster of contractile injection systems (CISs). Together, our findings expand the known diversity of CISs' structures and functions, and lay the groundwork for engineering customisable protein delivery platforms.
External links	Nat Commun / PubMed:41991534
Methods	EM (single particle) / EM (helical sym.)
Resolution	2.28 - 3.4 Å
Structure data	53855 9r9a EMDB-53855, PDB-9r9a: CryoEM structure of a membrane protein associated with a contractile injection in Salmonella enterica subspecies salamae Method: EM (single particle) / Resolution: 2.6 Å 53857 9r9h EMDB-53857, PDB-9r9h: Cryo-EM structure of an extracellular contractile injection system in Salmonella enterica subspecies salamae, the sheath and inner tube module in extended state. Method: EM (helical sym.) / Resolution: 2.28 Å 53858 9r9i EMDB-53858, PDB-9r9i: Cryo-EM structure of an extracellular contractile injection system in Salmonella enterica subspecies salamae, the sheath in contracted state. Method: EM (helical sym.) / Resolution: 3.4 Å 53859 9r9n EMDB-53859, PDB-9r9n: Cryo-EM structure of a contractile injection system in Salmonella enterica subspecies Salamae, the cap portion in extended state. Method: EM (single particle) / Resolution: 2.86 Å 53919 9rce EMDB-53919, PDB-9rce: Cryo-EM structure of a contractile injection system in Salmonella enterica subspecies Salamae, the baseplate portion in extended state. Method: EM (single particle) / Resolution: 2.86 Å
Source	salmonella enterica subsp. salamae (bacteria)
Keywords	MEMBRANE PROTEIN / Hydrolase / transmembrane / contractile injection system / CONTRACTILE PROTEIN / Salmonella enterica subspecies salamae / Contractile injections system / contractile injection system Salmonella