EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular insights into the capsular polysaccharide transporter Wza-Wzc complex.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Page 1436, Year 2026
掲載日	2026年2月5日
著者	Biao Yuan / Christian Sieben / Prateek Raj / Tina Rietschel / Rory Hennell James / Anja Gatzemeier / Lothar Jänsch / Thomas C Marlovits / Dirk W Heinz /
PubMed 要旨	Capsular polysaccharides (CPS) are key virulence determinants, constituting the protective capsule that surrounds bacterial pathogens. Here, we present the complete cryo-EM structure of Gram-negative ...Capsular polysaccharides (CPS) are key virulence determinants, constituting the protective capsule that surrounds bacterial pathogens. Here, we present the complete cryo-EM structure of Gram-negative bacterial CPS secretion machinery formed by the E. coli K12 Wza-Wzc complex. The structure reveals an elongated, continuous channel spanning the entire envelope that facilitates CPS secretion. Multiple structural snapshots of the ADP-bound Wza-Wzc complex capture intermediate conformations of the double membrane assembly, highlighting its remarkable intrinsic dynamics. In-depth analysis of the isolated Wza translocon and Wzc co-polymerase, reveals mechanistic details of both complex formation and CPS transport. We further uncover the jellyroll domain of Wzc as a CPS-binding module, likely guiding CPS repeat units into a proposed Wzc-Wzy polymerization platform. Collectively, this work provides structural and functional insights into CPS synthesis and transport, advancing our understanding of bacterial capsule formation and virulence mechanisms.
リンク	Nat Commun / PubMed:41644548 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 6.7 Å
構造データ	53598 9r60 EMDB-53598, PDB-9r60: CPS translocon Wza_C1 手法: EM (単粒子) / 解像度: 3.0 Å 53599 9r61 EMDB-53599, PDB-9r61: CPS translocon Wza_C8 手法: EM (単粒子) / 解像度: 2.7 Å 53600 9r62 EMDB-53600, PDB-9r62: CPS co-polymerase Wzc_C1 手法: EM (単粒子) / 解像度: 3.4 Å 53601 9r63 EMDB-53601, PDB-9r63: CPS co-polymerase Wzc_C4 手法: EM (単粒子) / 解像度: 3.2 Å 53602 9r64 EMDB-53602, PDB-9r64: CPS secretion pathway Wza-Wzc_C1 (Conf 0) 手法: EM (単粒子) / 解像度: 3.5 Å 53603 9r65 EMDB-53603, PDB-9r65: CPS secretion pathway Wza-Wzc_C8 (Conf 0) 手法: EM (単粒子) / 解像度: 3.2 Å 53604 9r66 EMDB-53604, PDB-9r66: CPS secretion pathway Wza-Wzc_C1 (Conf 1) 手法: EM (単粒子) / 解像度: 3.8 Å 53605 9r67 EMDB-53605, PDB-9r67: CPS secretion pathway Wza-Wzc_C8 (Conf 1) 手法: EM (単粒子) / 解像度: 3.5 Å 53606 9r68 EMDB-53606, PDB-9r68: CPS secretion pathway Wza-Wzc_C1 (Conf 2) 手法: EM (単粒子) / 解像度: 5.8 Å 53607 9r69 EMDB-53607, PDB-9r69: CPS secretion pathway Wza-Wzc_C8 (Conf 2) 手法: EM (単粒子) / 解像度: 4.3 Å 53608 9r6a EMDB-53608, PDB-9r6a: CPS secretion pathway Wza-Wzc (Conf 3) 手法: EM (単粒子) / 解像度: 6.3 Å 53609 9r6b EMDB-53609, PDB-9r6b: CPS secretion pathway Wza-Wzc (Conf 4) 手法: EM (単粒子) / 解像度: 4.6 Å 53610 9r6c EMDB-53610, PDB-9r6c: CPS secretion pathway Wza-Wzc (Conf 5) 手法: EM (単粒子) / 解像度: 6.7 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM ChemComp-MG*: Unknown entry
由来	escherichia coli k-12 (大腸菌)
キーワード	TRANSPORT PROTEIN / capsular polysaccharide / Capsular polysaccharide co-polymerase / Capsular polysaccharide secretion pathway / CPS transporter