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-Structure paper
| タイトル | Alphafold 3-guided insights into the Importinβ: Importin7 heterodimer interaction and its binding to histone H1. |
|---|---|
| ジャーナル・号・ページ | Structure, Vol. 34, Issue 3, Page 414-425.e4, Year 2026 |
| 掲載日 | 2026年3月5日 |
著者 | Piotr Neumann / Olexandr Dybkov / Henning Urlaub / Ralf Ficner / Achim Dickmanns / ![]() |
| PubMed 要旨 | The nuclear import of H1 linker histones is facilitated by a heterodimer of the transport receptors Importinβ (Impβ) and Importin7 (Imp7). The interaction between them is mediated by a stretch of C- ...The nuclear import of H1 linker histones is facilitated by a heterodimer of the transport receptors Importinβ (Impβ) and Importin7 (Imp7). The interaction between them is mediated by a stretch of C-terminal residues of Imp7 essential also for Imp7 activation by Impβ. An Impβ:Imp7:H1 complex model was predicted by Alphafold3 and validated using cross-linking data, isothermal titration calorimetry, and pull-down experiments, providing robust support for its accuracy. This model positions the H1 globular domain within the central cavity of Imp7. Refinement of this atomic model against a published cryo-electron microscopy (cryo-EM) map demonstrated significantly improved correspondence compared to the earlier interpretation, which placed the H1 globular domain within Impβ. This enhanced structural consistency further substantiates the accuracy of the AI-driven prediction. Moreover, a detailed analysis confirmed the extended C-terminal stretch of Imp7 harboring a nucleoporin-like binding (NlB) region with two FXFG-like nucleoporin motifs interacting with the outer surface of Impβ. |
リンク | Structure / PubMed:41529687 |
| 手法 | EM (単粒子) |
| 解像度 | 6.2 - 7.5 Å |
| 構造データ | ![]() PDB-9qej: ![]() PDB-9qf0: |
| 由来 |
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キーワード | TRANSPORT PROTEIN / Transport / Importin 7 / Importin Beta / Histone 1.0 |
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