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-Structure paper
| タイトル | Structure of a type II SMC Wadjet complex from Neobacillus vireti. |
|---|---|
| ジャーナル・号・ページ | Structure, Vol. 33, Issue 9, Page 1498-11509.e5, Year 2025 |
| 掲載日 | 2025年9月4日 |
著者 | Florian Roisné-Hamelin / Hon Wing Liu / Stephan Gruber / ![]() |
| PubMed 要旨 | Structural maintenance of chromosome complexes are essential DNA-folding motors that facilitate critical cellular functions, including chromosome segregation and DNA repair. Wadjet systems are ...Structural maintenance of chromosome complexes are essential DNA-folding motors that facilitate critical cellular functions, including chromosome segregation and DNA repair. Wadjet systems are prokaryotic SMC complexes specialized in cellular immunity against plasmids. Type I Wadjet systems restrict plasmids via a DNA extrusion-cleavage reaction. Two other Wadjet types (II and III) have also been identified, however, their molecular characteristics are unclear. Here, we reconstituted a representative type II Wadjet system from Neobacillus vireti. We show that this system shares substrate selection and cleavage properties with type I but exhibits distinctive structural features, including a long elbow-distal coiled coil, a channel-less hinge, and a tandem KITE subunit. These features help identify the common and distinguishing architectural elements in the family of Wadjet systems and raise intriguing questions about the evolution of prokaryotic SMC complexes. |
リンク | Structure / PubMed:40628265 |
| 手法 | EM (単粒子) |
| 解像度 | 3.5 - 6.71 Å |
| 構造データ | EMDB-53035, PDB-9qe0: ![]() EMDB-53036: Neobacillus vireti Wadjet-II JetC hinge EMDB-53037, PDB-9qe1: |
| 化合物 | ![]() ChemComp-ADP: |
| 由来 |
|
キーワード | DNA BINDING PROTEIN / SMC complexes / Wadjet / JetABCD / DNA loop extrusion |
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neobacillus vireti lmg 21834 (バクテリア)
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