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TitleHigh-Affinity, Structure-Validated and Selective Macrocyclic Peptide Tools for Chemical Biology Studies of Huntingtin.
Journal, issue, pagesbioRxiv, Year 2025
Publish dateAug 6, 2025
AuthorsEsther Wolf / Rebeka Fanti / Tatsuya Ikenoue / Justin C Deme / Swati Balakrishnan / Brandon A Keith / Matthew G Alteen / Renu Chandrasekaran / Manisha Yadav / Ritika Bhajiawala / Suzanne Ackloo / Jia Feng / Mahmoud A Pouladi / Aled M Edwards / Derek Wilson / Susan M Lea / Hiroaki Suga / Rachel J Harding /
PubMed AbstractHuntington's disease (HD) is a fatal neurodegenerative disorder caused by a CAG repeat expansion in the () gene, with no disease-modifying therapies currently available. The precise molecular ...Huntington's disease (HD) is a fatal neurodegenerative disorder caused by a CAG repeat expansion in the () gene, with no disease-modifying therapies currently available. The precise molecular function of the HTT protein is unclear, and the lack of selective chemical tools has limited functional studies. We have identified and characterized macrocyclic peptide binders targeting HTT. These binders exhibit low-nanomolar affinity and engage distinct HTT and HTT-HAP40 interfaces, as revealed by hydrogen-deuterium exchange mass spectrometry and cryo-electron microscopy. Chemoproteomics confirmed selective binding in cell extracts from wildtype but not HTT-null cell lines. HAP40 consistently and stoichiometrically co-purified with HTT across cell lines, including with HTT variants containing different CAG repeat lengths, highlighting the broad presence of the HTT-HAP40 complex.
External linksbioRxiv / PubMed:41030958 / PubMed Central
MethodsEM (single particle)
Resolution2.1 - 2.3 Å
Structure data

71743

9pmw

EMDB-71743, PDB-9pmw:
Structure of HTTQ23-HAP40 complex bound to macrocycles HHL1, HD4 and HL2
Method: EM (single particle) / Resolution: 2.1 Å

71744

9pn0

EMDB-71744, PDB-9pn0:
Structure of HTTQ23-HAP40 complex bound to macrocycles HHD3, HD4 and HL2
Method: EM (single particle) / Resolution: 2.3 Å

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsPEPTIDE BINDING PROTEIN / Huntingtin / Macrocycles / Polyglutamine expansion

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