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Structure paper

TitleStructural basis for regulating lipopolysaccharide transmembrane transport
Journal, issue, pagesTo Be Published
Publish dateJun 17, 2025 (structure data deposition date)
AuthorsTaylor RJ / Pahil KS / Walsh Jr RM / Kahne DE
External linksSearch PubMed
MethodsEM (single particle)
Resolution3.1 - 9.4 Å
Structure data

EMDB-71291: Raw consensus map of the E coli lipopolysaccharide transport bridge
Method: EM (single particle) / Resolution: 4.13 Å

EMDB-71292: Constituent EM map: Focused refinement LptADE of outer membrane and periplasmic portions of the E. coli lipopolysaccharide transport bridge
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-71293: Constituent EM map: Focused refinement of inner membrane portions of the E. coli lipopolysaccharide transport bridge LptBFGC
Method: EM (single particle) / Resolution: 4.06 Å

EMDB-71294: Constituent EM map: Outer membrane and periplasmic portions of the lipopolysaccharide transport bridge from E coli
Method: EM (single particle) / Resolution: 3.39 Å

EMDB-71296: Constituent EM map: Focused refinement inner membrane portion LptBFGC of the lipopolysaccharide transport bridge from E. coli in complex with lipopolysaccharide
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-71297: Consensus map of lipopolysaccharide transport bridge LptBFGCADE from E. coli in complex with lipopolysaccharide
Method: EM (single particle) / Resolution: 3.72 Å

EMDB-71298, PDB-9p52:
LptBFGCA from Escherichia coli
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-71347, PDB-9p7m:
LPS transport bridge LptBFGCADE from Escherichia coli
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-71350, PDB-9p7p:
LPS transport bridge LptBFGCADE from Escherichia coli in complex with lipopolysaccharide
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-76425: Constituent EM map: Focused refinement LptADE of outer membrane and periplasmic portions of the E. coli lipopolysaccharide transport bridge, low resolution structure
Method: EM (single particle) / Resolution: 9.4 Å

EMDB-76426: Constituent EM map: Focused refinement of inner membrane portions of the E. coli lipopolysaccharide transport bridge LptBFGC, low resolution structure
Method: EM (single particle) / Resolution: 7.9 Å

EMDB-76427: Consensus map: Low resolution structure of LPS transport bridge LptBFGCADE from Escherichia coli
Method: EM (single particle) / Resolution: 9.4 Å

EMDB-76428: Low resolution composite map of LPS transport bridge LptBFGCADE from Escherichia coli
Method: EM (single particle) / Resolution: 8.5 Å

EMDB-76430: Constituent EM map: Focused refinement LptADE of outer membrane and periplasmic portions of the E. coli lipopolysaccharide transport bridge in complex with ATP
Method: EM (single particle) / Resolution: 7.0 Å

EMDB-76431: Consensus map - Catalytically inactive LPS transport bridge LptBFGCADE from Escherichia coli bound to ATP
Method: EM (single particle) / Resolution: 8.1 Å

EMDB-76432: Constituent EM map: Focused refinement of inner membrane portions of the E. coli lipopolysaccharide transport bridge LptBFGC in complex with ATP
Method: EM (single particle) / Resolution: 7.4 Å

EMDB-76433: Catalytically inactive LPS transport bridge LptBFGCADE from Escherichia coli bound to ATP
Method: EM (single particle) / Resolution: 7.0 Å

Chemicals

ChemComp-JSG:
(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-5-[(2~{S},3~{S},4~{R},5~{R},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{R},5~{S},6~{R})-6-[(1~{S})-2-[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-1-oxidanyl-ethyl]-3,4-bis(oxidanyl)-5-phosphonooxy-oxan-2-yl]oxy-3-oxidanyl-5-phosphonooxy-oxan-2-yl]oxy-2-carboxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-5-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanyltetradecanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid

Source
  • escherichia coli (E. coli)
KeywordsLIPID TRANSPORT / Complex / Lipopolysaccharide / ABC / ATP binding cassette / transporter / bridge / intermembrane

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