EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for protein-free catalysis by ribonuclease P ribozyme.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Year 2026
掲載日	2026年4月15日
著者	Yun-Tzai Lee / Maximilia F S Degenhardt / Ilias Skeparnias / Szu-Yun Chen / Bapurao A Bhoge / Sergey G Tarasov / Marzena A Dyba / Jinwei Zhang / Jason R Stagno / Yun-Xing Wang /
PubMed 要旨	Ribonuclease P (RNase P) is an essential metallonuclease found in all three domains of life. However, the structural basis for the ancient RNase P RNA component acting alone as a ribozyme and ...Ribonuclease P (RNase P) is an essential metallonuclease found in all three domains of life. However, the structural basis for the ancient RNase P RNA component acting alone as a ribozyme and catalytic metal-ion chemistry remains unknown. We report a series of cryo-EM structures, at resolutions of 2.8-3.5 Å, of the Geobacillus stearothermophilus RNase P aporibozyme (apoE) in various states of the catalytic cycle. The formation of both the tetraloop/tetraloop-receptor interaction and the interdigitated double T-loop motif in the substrate-specificity domain facilitates substrate binding. The apoE uses two metal ions for catalysis, suggesting a catalytic mechanism and evolutionary importance of the RNase P ribozyme to function without its protein component. Together, our data portray the regulatory RNA-RNA interfaces, dynamic structures, and cation traffic that confer function to a trans-acting ribozyme.
リンク	Nat Commun / PubMed:41986363 / PubMed Central
手法	EM (単粒子)
解像度	2.78 - 3.46 Å
構造データ	70888 9ov3 EMDB-70888, PDB-9ov3: Structure of Geobacillus stearothermophilus RNase P ribozyme 手法: EM (単粒子) / 解像度: 3.19 Å 70891 9ov6 EMDB-70891, PDB-9ov6: Structure of Geobacillus stearothermophilus RNase P ribozyme sub-conformation 1 手法: EM (単粒子) / 解像度: 3.46 Å 70892 9ov7 EMDB-70892, PDB-9ov7: Structure of Geobacillus stearothermophilus RNase P ribozyme sub-conformation 2 手法: EM (単粒子) / 解像度: 3.27 Å 70893 9ov8 EMDB-70893, PDB-9ov8: Structure of Geobacillus stearothermophilus RNase P ribozyme sub-conformation 3 手法: EM (単粒子) / 解像度: 3.2 Å 70896 9ovb EMDB-70896, PDB-9ovb: Structure of Geobacillus stearothermophilus RNase P ribozyme in 5 mM Mg2+ 手法: EM (単粒子) / 解像度: 3.21 Å 70897 9ovc EMDB-70897, PDB-9ovc: Structure of Geobacillus stearothermophilus RNase P ribozyme in 10 mM Mg2+ 手法: EM (単粒子) / 解像度: 2.97 Å 70933 9owj EMDB-70933, PDB-9owj: Structure of Geobacillus stearothermophilus RNase P ribozyme in complex with precursor tRNA in 5 mM Ca2+ 手法: EM (単粒子) / 解像度: 2.94 Å 70935 9owl EMDB-70935, PDB-9owl: Structure of Geobacillus stearothermophilus RNase P ribozyme in complex with mature tRNA in 5 mM Ca2+ 手法: EM (単粒子) / 解像度: 2.99 Å 70936 9owm EMDB-70936, PDB-9owm: Structure of Geobacillus stearothermophilus RNase P ribozyme in complex with mature tRNA in 10 mM Ca2+ 手法: EM (単粒子) / 解像度: 2.95 Å 70937 9own EMDB-70937, PDB-9own: Structure of Geobacillus stearothermophilus RNase P ribozyme in complex with precursor tRNA with non-complementary 5' leader (Consensus) 手法: EM (単粒子) / 解像度: 2.86 Å 70940 9owq EMDB-70940, PDB-9owq: Structure of Geobacillus stearothermophilus RNase P ribozyme in complex with precursor tRNA with loop-back 5' leader 手法: EM (単粒子) / 解像度: 2.78 Å 70994 9oy2 EMDB-70994, PDB-9oy2: Structure of Geobacillus stearothermophilus RNase P ribozyme tetraloop mutant (sub-conformation 1) 手法: EM (単粒子) / 解像度: 3.14 Å 70995 9oy3 EMDB-70995, PDB-9oy3: Structure of Geobacillus stearothermophilus RNase P ribozyme tetraloop mutant (sub-conformation 2) 手法: EM (単粒子) / 解像度: 3.13 Å 70996 9oy4 EMDB-70996, PDB-9oy4: Structure of Geobacillus stearothermophilus RNase P ribozyme tetraloop mutant (sub-conformation 3) 手法: EM (単粒子) / 解像度: 3.11 Å
化合物	ChemComp-CA: Unknown entry ChemComp-MG: Unknown entry
由来	geobacillus stearothermophilus (バクテリア)
キーワード	RNA / ribozyme / RNase P / RNase P.