EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Distinct structural features of Pseudomonas aeruginosa ATP synthase revealed by cryo-electron microscopy.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Page 406, Year 2025
掲載日	2025年12月9日
著者	Meghna Sobti / Adam P Gunn / Simon H J Brown / Lauren Zavan / Vesper M Fraunfelter / Amanda L Wolfe / Christopher A McDevitt / P Ryan Steed / Alastair G Stewart /
PubMed 要旨	FF ATP synthase is the ubiquitous enzyme that synthesizes cellular ATP by coupling proton-motive force with rotational catalysis. Structural differences between prokaryotic and eukaryotic ATP ...FF ATP synthase is the ubiquitous enzyme that synthesizes cellular ATP by coupling proton-motive force with rotational catalysis. Structural differences between prokaryotic and eukaryotic ATP synthases offer potential targets for antimicrobial development. Here, we present the 2.0-2.4 Å resolution cryo-electron microscopy structures of the ATP synthase from Pseudomonas aeruginosa, an opportunistic bacterial pathogen capable of causing serious infections in humans. Our structures identify two distinctive features of this species' enzyme: a distinct binding site for the inhibitory ε subunit, and a coordinated metal ion capping the cytoplasmic proton channel. Lower-resolution maps of the enzyme following incubation with MgATP showed conformational rearrangements of the ε subunit during activation. Visualization of bound water molecules in the periplasmic half-channel supports a Grotthuss proton-transfer mechanism. Focused classification of the F motor resolves distinct ~11° sub-steps in the c-ring, corresponding to protonation and deprotonation events. Functional analyses show that modifications to either the ε subunit or the metal binding site influence ATP synthesis and hydrolysis. Mass spectrometry analyses suggests that the physiological metal within the complex is zinc. Collectively, these findings define structural features of P. aeruginosa ATP synthase that could serve as targets for antimicrobial therapeutics.
リンク	Nat Commun / PubMed:41366214 / PubMed Central
手法	EM (単粒子)
解像度	2.0 - 3.57 Å
構造データ	49999 9o19 EMDB-49999, PDB-9o19: Pseudomonas aeruginosa ATPase State1 手法: EM (単粒子) / 解像度: 2.0 Å 70000 9o1a EMDB-70000, PDB-9o1a: Pseudomonas aeruginosa ATPase State1 F1Fo focused 手法: EM (単粒子) / 解像度: 2.77 Å 70001 9o1b EMDB-70001, PDB-9o1b: Pseudomonas aeruginosa ATPase State1 Fo focused 手法: EM (単粒子) / 解像度: 2.37 Å 70002 9o1c EMDB-70002, PDB-9o1c: Pseudomonas aeruginosa ATPase State2 手法: EM (単粒子) / 解像度: 2.05 Å 70003 9o1d EMDB-70003, PDB-9o1d: Pseudomonas aeruginosa ATPase State2a F1Fo focused 手法: EM (単粒子) / 解像度: 3.23 Å 70004 9o1e EMDB-70004, PDB-9o1e: Pseudomonas aeruginosa ATPase State2a Fo focused 手法: EM (単粒子) / 解像度: 2.59 Å 70005 9o1f EMDB-70005, PDB-9o1f: Pseudomonas aeruginosa ATPase State2b F1Fo focused 手法: EM (単粒子) / 解像度: 2.76 Å 70006 9o1g EMDB-70006, PDB-9o1g: Pseudomonas aeruginosa ATPase State2b Fo focused 手法: EM (単粒子) / 解像度: 2.66 Å 70007 9o1h EMDB-70007, PDB-9o1h: Pseudomonas aeruginosa ATPase State3 手法: EM (単粒子) / 解像度: 2.17 Å 70009 9o1j EMDB-70009, PDB-9o1j: Pseudomonas aeruginosa ATPase State3 F1Fo focused 手法: EM (単粒子) / 解像度: 3.02 Å 70010 9o1k EMDB-70010, PDB-9o1k: Pseudomonas aeruginosa ATPase State3 Fo focused 手法: EM (単粒子) / 解像度: 3.01 Å EMDB-71967: Pseudomonas aeruginosa ATPase State2 with 10mM MgATP "Up" 手法: EM (単粒子) / 解像度: 3.37 Å EMDB-71968: Pseudomonas aeruginosa ATPase State2 with 10mM MgATP "Down" 手法: EM (単粒子) / 解像度: 3.57 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-HOH: WATER ChemComp-ZN: Unknown entry
由来	pseudomonas aeruginosa (緑膿菌)
キーワード	ELECTRON TRANSPORT / ATPase / energy / Cryo-EM / single particle