EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Archaeal and eukaryotic MCM rings sequentially melt DNA for replication initiation.
ジャーナル・号・ページ	Nat Commun, Vol. 17, Issue 1, Year 2026
掲載日	2026年3月31日
著者	Sanaz Rasouli / Alexander Myasnikov / Eric J Enemark /
PubMed 要旨	DNA replication initiation requires local melting of fully base-paired DNA for a helicase to gain a foothold and initiate processive DNA unwinding. In eukaryotes and archaea, the helicase engine is ...DNA replication initiation requires local melting of fully base-paired DNA for a helicase to gain a foothold and initiate processive DNA unwinding. In eukaryotes and archaea, the helicase engine is the hexameric ring minichromosome maintenance (MCM) complex. In eukaryotes, a defined biochemical sequence assembles two Cdc45-MCM-GINS (CMG) complexes that provide limited DNA unwinding as the species that immediately precedes extensive unwinding. A prior structure revealed how MCM subunits interact with this form of DNA, but the atomic progression from undistorted DNA to this melted DNA species is unknown. Here, we present a sequential DNA melting mechanism determined by snapshots of an archaeal MCM ring with DNA in varying degrees of melting. In this mechanism, successive ATP-binding at MCM ATPase sites drives sequential discrete DNA melting steps mediated a specific MCM aromatic residue. Analysis of eukaryotic structures shows loaded MCM rings principally adopt only two molecular arrangements at the ATPase: one that does not melt DNA and one tuned to melt DNA with equivalent aromatic residues, indicating a universal sequential mechanism melts DNA in archaea and eukaryotes for replication initiation.
リンク	Nat Commun / PubMed:41916983 / PubMed Central
手法	EM (単粒子)
解像度	2.45 - 4.04 Å
構造データ	49806 9nuh EMDB-49806, PDB-9nuh: SsoPfMCM:DNA class 1a from merged particles 手法: EM (単粒子) / 解像度: 2.92 Å 49807 9nui EMDB-49807, PDB-9nui: SsoPfMCM:DNA class 1b from merged particles 手法: EM (単粒子) / 解像度: 3.24 Å 49808 9nuj EMDB-49808, PDB-9nuj: SsoPfMCM:DNA class 1c from merged particles 手法: EM (単粒子) / 解像度: 3.44 Å 49809 9nuk EMDB-49809, PDB-9nuk: SsoPfMCM:DNA class 2a from merged particles 手法: EM (単粒子) / 解像度: 2.7 Å 49810 9nul EMDB-49810, PDB-9nul: SsoPfMCM:DNA class 2b from merged particles 手法: EM (単粒子) / 解像度: 2.5 Å 49811 9num EMDB-49811, PDB-9num: SsoPfMCM:DNA class 3 from merged particles 手法: EM (単粒子) / 解像度: 3.67 Å 49812 9nun EMDB-49812, PDB-9nun: SsoPfMCM:DNA class 1a from DNA 1 手法: EM (単粒子) / 解像度: 3.36 Å 49813 9nuo EMDB-49813, PDB-9nuo: SsoPfMCM:DNA class 1b from DNA 1 手法: EM (単粒子) / 解像度: 3.58 Å 49814 9nup EMDB-49814, PDB-9nup: SsoPfMCM:DNA class 1c from DNA 1 手法: EM (単粒子) / 解像度: 3.9 Å 49815 9nuq EMDB-49815, PDB-9nuq: SsoPfMCM:DNA class 2a from DNA 1 手法: EM (単粒子) / 解像度: 3.13 Å 49816 9nur EMDB-49816, PDB-9nur: SsoPfMCM:DNA class 2b from DNA 1 手法: EM (単粒子) / 解像度: 2.99 Å 49817 9nus EMDB-49817, PDB-9nus: SsoPfMCM:DNA class 3 from DNA 1 手法: EM (単粒子) / 解像度: 4.04 Å 49818 9nut EMDB-49818, PDB-9nut: SsoPfMCM:DNA class 1a from DNA 2 手法: EM (単粒子) / 解像度: 2.93 Å 49819 9nuu EMDB-49819, PDB-9nuu: SsoPfMCM:DNA class 1b from DNA 2 手法: EM (単粒子) / 解像度: 3.38 Å 49820 9nuv EMDB-49820, PDB-9nuv: SsoPfMCM:DNA class 1c from DNA 2 手法: EM (単粒子) / 解像度: 3.51 Å 49821 9nuw EMDB-49821, PDB-9nuw: SsoPfMCM:DNA class 2a from DNA 2 手法: EM (単粒子) / 解像度: 2.65 Å 49822 9nux EMDB-49822, PDB-9nux: SsoPfMCM:DNA class 2b from DNA 2 手法: EM (単粒子) / 解像度: 2.45 Å 49823 9nuy EMDB-49823, PDB-9nuy: SsoPfMCM:DNA class 3 from DNA 2 手法: EM (単粒子) / 解像度: 3.52 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-HOH: WATER ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, エネルギー貯蔵分子類似体YM
由来	saccharolobus solfataricus p2 (古細菌) pyrococcus furiosus dsm 3638 (古細菌) synthetic construct (人工物)
キーワード	REPLICATION / HYDROLASE/DNA / DNA replication / helicase / MCM / HYDROLASE- DNA complex / HYDROLASE-DNA complex