Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Archaeal and eukaryotic MCM rings sequentially melt DNA for replication initiation.
Journal, issue, pages	Nat Commun, Vol. 17, Issue 1, Year 2026
Publish date	Mar 31, 2026
Authors	Sanaz Rasouli / Alexander Myasnikov / Eric J Enemark /
PubMed Abstract	DNA replication initiation requires local melting of fully base-paired DNA for a helicase to gain a foothold and initiate processive DNA unwinding. In eukaryotes and archaea, the helicase engine is ...DNA replication initiation requires local melting of fully base-paired DNA for a helicase to gain a foothold and initiate processive DNA unwinding. In eukaryotes and archaea, the helicase engine is the hexameric ring minichromosome maintenance (MCM) complex. In eukaryotes, a defined biochemical sequence assembles two Cdc45-MCM-GINS (CMG) complexes that provide limited DNA unwinding as the species that immediately precedes extensive unwinding. A prior structure revealed how MCM subunits interact with this form of DNA, but the atomic progression from undistorted DNA to this melted DNA species is unknown. Here, we present a sequential DNA melting mechanism determined by snapshots of an archaeal MCM ring with DNA in varying degrees of melting. In this mechanism, successive ATP-binding at MCM ATPase sites drives sequential discrete DNA melting steps mediated a specific MCM aromatic residue. Analysis of eukaryotic structures shows loaded MCM rings principally adopt only two molecular arrangements at the ATPase: one that does not melt DNA and one tuned to melt DNA with equivalent aromatic residues, indicating a universal sequential mechanism melts DNA in archaea and eukaryotes for replication initiation.
External links	Nat Commun / PubMed:41916983 / PubMed Central
Methods	EM (single particle)
Resolution	2.45 - 4.04 Å
Structure data	49806 9nuh EMDB-49806, PDB-9nuh: SsoPfMCM:DNA class 1a from merged particles Method: EM (single particle) / Resolution: 2.92 Å 49807 9nui EMDB-49807, PDB-9nui: SsoPfMCM:DNA class 1b from merged particles Method: EM (single particle) / Resolution: 3.24 Å 49808 9nuj EMDB-49808, PDB-9nuj: SsoPfMCM:DNA class 1c from merged particles Method: EM (single particle) / Resolution: 3.44 Å 49809 9nuk EMDB-49809, PDB-9nuk: SsoPfMCM:DNA class 2a from merged particles Method: EM (single particle) / Resolution: 2.7 Å 49810 9nul EMDB-49810, PDB-9nul: SsoPfMCM:DNA class 2b from merged particles Method: EM (single particle) / Resolution: 2.5 Å 49811 9num EMDB-49811, PDB-9num: SsoPfMCM:DNA class 3 from merged particles Method: EM (single particle) / Resolution: 3.67 Å 49812 9nun EMDB-49812, PDB-9nun: SsoPfMCM:DNA class 1a from DNA 1 Method: EM (single particle) / Resolution: 3.36 Å 49813 9nuo EMDB-49813, PDB-9nuo: SsoPfMCM:DNA class 1b from DNA 1 Method: EM (single particle) / Resolution: 3.58 Å 49814 9nup EMDB-49814, PDB-9nup: SsoPfMCM:DNA class 1c from DNA 1 Method: EM (single particle) / Resolution: 3.9 Å 49815 9nuq EMDB-49815, PDB-9nuq: SsoPfMCM:DNA class 2a from DNA 1 Method: EM (single particle) / Resolution: 3.13 Å 49816 9nur EMDB-49816, PDB-9nur: SsoPfMCM:DNA class 2b from DNA 1 Method: EM (single particle) / Resolution: 2.99 Å 49817 9nus EMDB-49817, PDB-9nus: SsoPfMCM:DNA class 3 from DNA 1 Method: EM (single particle) / Resolution: 4.04 Å 49818 9nut EMDB-49818, PDB-9nut: SsoPfMCM:DNA class 1a from DNA 2 Method: EM (single particle) / Resolution: 2.93 Å 49819 9nuu EMDB-49819, PDB-9nuu: SsoPfMCM:DNA class 1b from DNA 2 Method: EM (single particle) / Resolution: 3.38 Å 49820 9nuv EMDB-49820, PDB-9nuv: SsoPfMCM:DNA class 1c from DNA 2 Method: EM (single particle) / Resolution: 3.51 Å 49821 9nuw EMDB-49821, PDB-9nuw: SsoPfMCM:DNA class 2a from DNA 2 Method: EM (single particle) / Resolution: 2.65 Å 49822 9nux EMDB-49822, PDB-9nux: SsoPfMCM:DNA class 2b from DNA 2 Method: EM (single particle) / Resolution: 2.45 Å 49823 9nuy EMDB-49823, PDB-9nuy: SsoPfMCM:DNA class 3 from DNA 2 Method: EM (single particle) / Resolution: 3.52 Å
Chemicals	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-HOH: WATER ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogueYM
Source	saccharolobus solfataricus p2 (archaea) pyrococcus furiosus dsm 3638 (archaea) synthetic construct (others)
Keywords	REPLICATION / HYDROLASE/DNA / DNA replication / helicase / MCM / HYDROLASE- DNA complex / HYDROLASE-DNA complex