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TitleStructure of an initiation complex mycobacterial helicase-nuclease AdnAB at a blunt double-strand break reveals locl melting that engages the 3' tracking strand at the ratchet pawl of the helicase motor
Journal, issue, pagesTo Be Published
Publish dateMar 11, 2025 (structure data deposition date)
AuthorsWarren GM / De la Cruz MJ / Goldgur Y / Meir A / Greene EC / Shuman S
External linksSearch PubMed
MethodsEM (single particle)
Resolution3.4 Å
Structure data

49626

9np6

EMDB-49626, PDB-9np6:
Cryo-EM structure of AdnA(D934A)-AdnB(D1014A) in complex with AMPPNP and blunt end DNA
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-SF4:
IRON/SULFUR CLUSTER

Source
  • Mycolicibacterium smegmatis (bacteria)
  • mycolicibacterium smegmatis mc2 155 (bacteria)
  • synthetic construct (others)
KeywordsISOMERASE/DNA / ATPase / helicase / DNA / ISOMERASE-DNA complex

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