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TitleStructure-based Enzyme Engineering of CCD1 to Improve Substrate Specificity for beta-ionone Biosynthesis
Journal, issue, pagesTo Be Published
Publish dateFeb 10, 2025 (structure data deposition date)
AuthorsSharma, D. / Xue, B. / Esque, E. / Bozonnet, S. / Ong, J.S. / Yew, W.S. / Remaud-Simeon, M. / Andre, I. / Chen, X.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution2.3 - 2.65 Å
Structure data

PDB-9luz:
Carotenoid Cleavage Dioxygenase 1 from Osmanthus fragrans
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-9m3e:
Carotenoid Cleavage Dioxygenase 1 variant from Osmanthus fragrans A191G E408Q
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-9m6z:
Carotenoid Cleavage Dioxygenase 1 variant from Osmanthus fragrans A191M E408Q
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

Chemicals

ChemComp-NI:
NICKEL (II) ION

ChemComp-SO4:
SULFATE ION

ChemComp-OXN:
OXTOXYNOL-10 / detergent*YM

ChemComp-HOH:
WATER

Source
  • osmanthus fragrans (sweet osmanthus)
KeywordsPLANT PROTEIN / Biosynthesis of ionone / CCD1

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