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TitleCnp1 N-terminal dynamics regulate L1 loop recognition by Mis15 to orchestrate kinetochore assembly in Schizosaccharomyces pombe.
Journal, issue, pagesJ Mol Cell Biol, Year 2025
Publish dateDec 26, 2025
AuthorsYujie Xiong / Yanze Jian / Yongliang Zhang / Min Zhang / Xuan Zhang / Kaiming Zhang / Chuanhai Fu / Tian Tian / Jianye Zang /
PubMed AbstractCentromeres are defined by the histone H3 variant CENP-A, which serve as the foundation for kinetochore assembly and ensure faithful chromosome segregation. CENP-A nucleosomes possess distinctive ...Centromeres are defined by the histone H3 variant CENP-A, which serve as the foundation for kinetochore assembly and ensure faithful chromosome segregation. CENP-A nucleosomes possess distinctive dynamic features, including flexible DNA ends at the entry/exit sites and a mobile N-terminal region, which are properties proposed to facilitate kinetochore assembly, yet the underlying molecular mechanisms remain elusive. Here, we present cryo-electron microscopy structures of Cnp1, the Schizosaccharomyces pombe (S. pombe) ortholog of CENP-A, alone and in complex with Mis15, the fission yeast ortholog of CENP-N. By integrating structural, biochemical, and molecular dynamics analyses, we demonstrate that the N-terminal region of Cnp1 regulates both DNA-end breathing and the conformational mobility of the L1 loop, a critical structural element for Mis15 recognition. Either enhanced dynamics caused by N-terminal deletion or reduced dynamics from targeted residue substitution disrupt Mis15 binding in vitro and impair its centromeric localization in vivo, thereby compromising the earliest steps of constitutive centromere-associated network assembly. Our findings establish the Cnp1 N-terminus as a dynamic allosteric modulator of chromatin architecture and reveal an L1 loop modulation mechanism that links nucleosome flexibility to kinetochore specification and chromosome segregation fidelity in fission yeast.
External linksJ Mol Cell Biol / PubMed:41453208
MethodsEM (single particle)
Resolution2.99 - 3.83 Å
Structure data

63344

9lrv

EMDB-63344, PDB-9lrv:
Cryo-EM structure of Fission yeast centromeric nucleosome Class 1
Method: EM (single particle) / Resolution: 2.99 Å

63345

9lrw

EMDB-63345, PDB-9lrw:
Cryo-EM structure of Fission yeast centromeric nucleosome Class 2
Method: EM (single particle) / Resolution: 3.04 Å

EMDB-63346: cryo-EM structure of Mis151-249-Cnp1 nucleosome complex
Method: EM (single particle) / Resolution: 3.83 Å

Source
  • schizosaccharomyces pombe (strain 972 / atcc 24843) (yeast)
  • Schizosaccharomyces pombe 972h- (yeast)
KeywordsNUCLEAR PROTEIN/DNA / Nucleosome dynamics / CENP-A / Cnp1 / kinetochore assembly / Chromosome Segregation / NUCLEAR PROTEIN-DNA complex

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