+検索条件
-Structure paper
| タイトル | Structural basis for Salmonella infection by two Microviridae phages. |
|---|---|
| ジャーナル・号・ページ | Commun Biol, Vol. 8, Issue 1, Page 1166, Year 2025 |
| 掲載日 | 2025年8月6日 |
著者 | Wanlong Hu / Zhengjie Liu / Yuming Wei / Qucheng Bian / Weiqi Lan / Chongzheng Fan / Jiaoyang Song / Qianqian Sun / Xiaojie Zhang / Yuqing Liu / Yan Gao / Yibao Chen / ![]() |
| PubMed 要旨 | The global resurgence of multidrug-resistant Salmonella species, responsible for millions of annual infections, underscores the urgent need for alternative antimicrobial strategies, such as phage ...The global resurgence of multidrug-resistant Salmonella species, responsible for millions of annual infections, underscores the urgent need for alternative antimicrobial strategies, such as phage therapy. Microviridae phages offer a promising model for studying phage-host interactions with their unique structural and infection mechanisms. Here, we identify two Microviridae phages, PJNS001 and PJNS002, with different host receptor dependencies, and determine their cryo-EM structures at 2.68 Å and 2.59 Å resolution, respectively. These icosahedral capsids with T = 1 symmetry exhibit a unique vertex reinforcement mechanism, stabilizing the viral assembly. The specific pentameric adaptations, coupled with DNA binding protein engagements and thermodynamic constraints, collectively preclude the formation of hybrid virions. Structural analysis and in situ visualization reveal spike protein features and host-attachment intermediates, informing host specificity. Together, these findings advance our understanding of Microviridae infection mechanisms and provide a structural framework for rational phage design against antibiotic-resistant pathogens. |
リンク | Commun Biol / PubMed:40770068 / PubMed Central |
| 手法 | EM (単粒子) / EM (サブトモグラム平均) |
| 解像度 | 2.59 - 32.3 Å |
| 構造データ | EMDB-62019, PDB-9k3m: ![]() EMDB-62020: The Map of PJNS002 spike protein G with Salmonella enterica LPS ![]() EMDB-62021: The STA map of PJNS001 attached on Salmonella outer membrane ![]() EMDB-62022: The STA map of PJNS002 attached on Salmonella outer membrane EMDB-62023, PDB-9k3n: |
| 由来 |
|
キーワード | VIRUS / structual proteins |
ムービー
コントローラー
構造ビューア
万見文献について



著者
リンク






microviridae (ウイルス)
キーワード