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TitleStructures and mechanisms of the ABC transporter ABCB1 from Arabidopsis thaliana.
Journal, issue, pagesStructure, Vol. 33, Issue 5, Page 903-915.e5, Year 2025
Publish dateMay 1, 2025
AuthorsQian Chen / Li Zhu / Sufen Zhang / Shuai Qiao / Zhong Jie Ding / Shao Jian Zheng / Jiangtao Guo / Nannan Su /
PubMed AbstractThe Arabidopsis thaliana auxin transporter ABCB1 plays a fundamental role in the regulation of plant growth and development. While its homolog ABCB19 was previously shown to transport ...The Arabidopsis thaliana auxin transporter ABCB1 plays a fundamental role in the regulation of plant growth and development. While its homolog ABCB19 was previously shown to transport brassinosteroids (BR), another class of essential hormones, the ability of ABCB1 to mediate BR transport has remained unexplored. In this study we show that ABCB1 also transports brassinosteroids with an in vitro brassinolide (BL) transport assay. Using single-particle cryo-electron microscopy, we determined ABCB1 structures in multiple inward-facing conformations in the apo state, ANP-bound state, BL-bound state, and the both BL- and ANP-bound state. BL binds to the large cavity of two transmembrane domains, inducing a slight conformational change. Additionally, we obtained the structure of ABCB1 in an outward-facing conformation. By comparing these different conformations, we elucidated the possible mechanism of hormone transport by ABCB1. These high-resolution structures help us to understand the structural basis for hormone recognition and transport mechanisms of ABCB1.
External linksStructure / PubMed:40101709
MethodsEM (single particle)
Resolution3.1 - 3.4 Å
Structure data

61827

9juj

EMDB-61827, PDB-9juj:
Structure of Arabidopsis thaliana ABCB1 in the inward-facing conformation
Method: EM (single particle) / Resolution: 3.4 Å

61828

9juk

EMDB-61828, PDB-9juk:
Structure of Arabidopsis thaliana ABCB1 with AMP-PNP bound in the inward-facing conformation
Method: EM (single particle) / Resolution: 3.3 Å

61829

9jul

EMDB-61829, PDB-9jul:
Structure of Arabidopsis thaliana ABCB1 with brassinolide bound in the inward-facing conformation
Method: EM (single particle) / Resolution: 3.1 Å

61830

9jum

EMDB-61830, PDB-9jum:
Structure of Arabidopsis thaliana ABCB1 with brassinolide and AMP-PNP bound in the inward-facing conformation
Method: EM (single particle) / Resolution: 3.1 Å

61831

9jun

EMDB-61831, PDB-9jun:
Structure of Arabidopsis thaliana ABCB1 in the inward-facing conformation under IAA condition
Method: EM (single particle) / Resolution: 3.4 Å

61832

9juo

EMDB-61832, PDB-9juo:
Structure of Arabidopsis thaliana ABCB1 with AMP-PNP bound in the inward-facing conformation under IAA condition
Method: EM (single particle) / Resolution: 3.1 Å

61833

9jup

EMDB-61833, PDB-9jup:
Structure of Arabidopsis thaliana ABCB1 with AMP-PNP bound in the outward-facing conformation
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-BLD:
Brassinolide

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSPORT PROTEIN / ABCB1 / auxin transport / brassinolide (BL) transport

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