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Structure paper

TitleA Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 64, Page e202424955-e202424955, Year 2025
Publish dateDec 3, 2024 (structure data deposition date)
AuthorsWalterspiel, F. / Ugarte-Uribe, B. / Weidenhausen, J. / Vincent, M. / Narayanasamy, K.K. / Dimitriadi, A. / Khan, A.U.M. / Fritsch, M. / Muller, C.W. / Zimmermann, T. / Deo, C.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:41131894
MethodsX-ray diffraction
Resolution2.4 Å
Structure data

PDB-9hkf:
X-Ray crystal structure of a photoswitchable HaloTag bound to JF635
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-PUJ:
(1E,3S)-1-{10-[2-carboxy-5-({2-[2-(hexyloxy)ethoxy]ethyl}carbamoyl)phenyl]-7-(3-fluoroazetidin-1-yl)-5,5-dimethyldibenz

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • rhodococcus rhodochrous (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / HaloTag / LOV domain / FMN / dye / photoswitch / psHaloTag / Light-oxygen-voltage-sensing domain / rhodamine

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