EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of AcMNPV nucleocapsid reveals DNA portal organization and packaging apparatus of circular dsDNA baculovirus.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 4844, Year 2025
掲載日	2025年5月24日
著者	Gregory Effantin / Eaazhisai Kandiah / Martin Pelosse /
PubMed 要旨	Baculoviruses are large DNA viruses found in nature propagating amongst insects and lepidoptera in particular. They have been studied for decades and are nowadays considered as invaluable ...Baculoviruses are large DNA viruses found in nature propagating amongst insects and lepidoptera in particular. They have been studied for decades and are nowadays considered as invaluable biotechnology tools used as biopesticides, recombinant expression systems or delivery vehicle for gene therapy. However, little is known about the baculovirus nucleocapsid assembly at a molecular level. Here, we solve the whole structure of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) nucleocapsid by applying cryo-electron microscopy (CryoEM) combined with de novo modelling and Alphafold predictions. Our structure completes prior observations and elucidates the intricate architecture of the apical cap, unravelling the organization of a DNA portal featuring intriguing symmetry mismatches between its core and vertex. The core, closing the capsid at the apex, holds two DNA helices of the viral genome tethered to Ac54 proteins. Different symmetry components at the apical cap and basal structure are constituted of the same building block, made of Ac101/Ac144, proving the versatility of this modular pair. The crown forming the portal vertex displays a C21 symmetry and contains, amongst others, the motor-like protein Ac66. Our findings support the viral portal to be involved in DNA packaging, probably in conjunction with other parts of a larger DNA packaging apparatus.
リンク	Nat Commun / PubMed:40413174 / PubMed Central
手法	EM (らせん対称) / EM (単粒子)
解像度	3.0 - 6.6 Å
構造データ	51771 9h1s EMDB-51771, PDB-9h1s: AcMNPV helical nucleocapsid 手法: EM (らせん対称) / 解像度: 3.0 Å 51791 9h2a EMDB-51791, PDB-9h2a: AcMNPV complete basal cap 手法: EM (単粒子) / 解像度: 5.2 Å 51792 9h2b EMDB-51792, PDB-9h2b: AcMNPV basal cap - C14 anchor complex only 手法: EM (単粒子) / 解像度: 4.1 Å 51793 9h2c EMDB-51793, PDB-9h2c: AcMNPV basal cap - C7 plug only 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-51794: AcMNPV apical cap - C2 core only 手法: EM (単粒子) / 解像度: 6.0 Å 51803 9h2h EMDB-51803, PDB-9h2h: AcMNPV apical cap - composite map of the C2 plug 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-51805: AcMNPV apical cap - C2 plug only -consensus 3D map 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-51806: AcMNPV apical cap - C2 plug only - focused map 1 手法: EM (単粒子) / 解像度: 6.1 Å EMDB-51807: AcMNPV apical cap - C2 plug focused map 2 手法: EM (単粒子) / 解像度: 6.5 Å 51808 9h2j EMDB-51808, PDB-9h2j: AcMNPV apical cap - C14 anchor complex only 手法: EM (単粒子) / 解像度: 4.7 Å 51809 9h2k EMDB-51809, PDB-9h2k: AcMNPV apical cap - C21 ring 手法: EM (単粒子) / 解像度: 3.5 Å
化合物	ChemComp-ZN: Unknown entry
由来	autographa californica nucleopolyhedrovirus (ウイルス)
キーワード	VIRUS / nucleocapsid / helical