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-Structure paper
| タイトル | The structure of the mammalian bornavirus polymerase complex. |
|---|---|
| ジャーナル・号・ページ | Nat Commun, Vol. 16, Issue 1, Page 7508, Year 2025 |
| 掲載日 | 2025年8月13日 |
著者 | Loïc Carrique / Franziska Günl / Adrian Deng / Jonathan M Grimes / Jeremy R Keown / ![]() |
| PubMed 要旨 | Borna disease virus 1 (BoDV-1) is a non-segmented RNA virus with one of the smallest known RNA virus genomes. BoDV-1 replicates in the nucleus of infected cells using a virally encoded polymerase ...Borna disease virus 1 (BoDV-1) is a non-segmented RNA virus with one of the smallest known RNA virus genomes. BoDV-1 replicates in the nucleus of infected cells using a virally encoded polymerase complex composed of the large protein and phosphoprotein. Here, we present the BoDV-1 polymerase complex at resolutions up to 2.8 Å, describing the fully ordered large polymerase protein bound to tetrameric phosphoprotein. The complex is maintained through the ordered C-terminal region of one copy of the phosphoprotein. Analysis of the model reveals a conserved methyltransferase domain, though key S-adenosyl methionine binding residues are missing. While no RNA is observed in our models, analysis of a sample under reaction conditions induces an opening and closing of the template entry and exit channels, respectively. Higher-order polymerase assemblies suggest oligomerisation as a conserved feature of negative strand RNA virus polymerases. We provide a molecular framework to investigate bornavirus replication and transcription. |
リンク | Nat Commun / PubMed:40804239 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.95 - 3.07 Å |
| 構造データ | EMDB-51765, PDB-9h1g: EMDB-51770, PDB-9h1q: EMDB-51785, PDB-9h1y: |
| 化合物 | ![]() ChemComp-ZN: |
| 由来 |
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キーワード | VIRAL PROTEIN / polymerase / phosphoprotein / bornavirus / RdRp |
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borna disease virus 1 (ウイルス)
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