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| Title | A broadly reactive ultralong bovine antibody that can determine the integrity of foot-and-mouth disease virus capsids. |
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| Journal, issue, pages | J Gen Virol, Vol. 105, Issue 10, Year 2024 |
| Publish date | Oct 18, 2024 |
 Authors | John D Clarke / Helen M E Duyvesteyn / Eva Perez-Martin / Undīne Latišenko / Claudine Porta / Kathleen V Humphreys / Abigail L Hay / Jingshan Ren / Elizabeth E Fry / Erwin van den Born / Bryan Charleston / Marie Bonnet-Di Placido / Raymond J Owens / David I Stuart / John A Hammond /   |
| PubMed Abstract | Foot-and-mouth disease vaccination using inactivated virus is suboptimal, as the icosahedral viral capsids often disassemble into antigenically distinct pentameric units during long-term storage, or ...Foot-and-mouth disease vaccination using inactivated virus is suboptimal, as the icosahedral viral capsids often disassemble into antigenically distinct pentameric units during long-term storage, or exposure to elevated temperature or lowered pH, and thus raise a response that is no longer protective. Furthermore, as foot-and-mouth disease virus (FMDV)'s seven serotypes are antigenically diverse, cross-protection from a single serotype vaccine is limited, and most existing mouse and bovine antibodies and camelid single-domain heavy chain-only antibodies are serotype-specific. For quality control purposes, there is a real need for pan-serotype antibodies that clearly distinguish between pentamer (12S) and protective intact FMDV capsid. To date, few cross-serotype bovine-derived antibodies have been reported in the literature. We identify a bovine antibody with an ultralong CDR-H3, Ab117, whose structural analysis reveals that it binds to a deep, hydrophobic pocket on the interior surface of the capsid via the CDR-H3. Main-chain and hydrophobic interactions provide broad serotype specificity. ELISA analysis confirms that Ab117 is a novel pan-serotype and conformational epitope-specific 12S reagent, suitable for assessing capsid integrity. |
 External links | J Gen Virol / PubMed:39422666 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 2.9 Å |
| Structure data | EMDB-51105, PDB-9g6v: |
| Source |
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 Keywords | VIRUS LIKE PARTICLE / disrupted / dissociated / VLP / internal epitope / pan-specific / ultralong |
bos taurus (domestic cattle)
foot-and-mouth disease virus sat 2