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TitleA VLP vaccine platform comprising the core protein of hepatitis B virus with N-terminal antigen capture.
Journal, issue, pagesInt J Biol Macromol, Vol. 305, Issue Pt 2, Page 141152, Year 2025
Publish dateFeb 15, 2025
AuthorsKaniz Fatema / Joseph S Snowden / Alexander Watson / Lee Sherry / Neil A Ranson / Nicola J Stonehouse / David J Rowlands /
PubMed AbstractNanoparticle presentation systems offer the potential to develop new vaccines rapidly in response to emerging diseases, a public health need that has become increasingly evident in the wake of the ...Nanoparticle presentation systems offer the potential to develop new vaccines rapidly in response to emerging diseases, a public health need that has become increasingly evident in the wake of the COVID-19 pandemic. Previously, we reported a nanoparticle scaffold system termed VelcroVax. This was constructed by insertion of a high affinity SUMO binding protein (Affimer), able to recognise a SUMO peptide tag, into the major immunodominant region of VLPs assembled from a tandem (fused dimer) form of hepatitis B virus (HBV) core protein (HBc). Here we describe an alternative form, termed N-VelcroVax, a VLP vaccine platform assembled from a monomeric HBc protein (N-anti-SUMO Affimer HBc 190) with the Affimer inserted at the N-terminus. In contrast to the tandem form of VelcroVax, N-VelcroVax VLPs were expressed well in E. coli. The VLPs effectively bound SUMO-tagged Junín virus glycoprotein, gp1 as assessed by structural and serological analyses. Cryo-EM characterisation of N-VelcroVax complexed with a SUMO-Junín gp1 showed continuous density attributable to the fused Affimer, in addition to evidence of target antigen capture. Collectively, these data suggest that N-VelcroVax has potential as a versatile next generation vaccine scaffold.
External linksInt J Biol Macromol / PubMed:39961558 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.5 Å
Structure data

50832

9fwe

EMDB-50832, PDB-9fwe:
N-VelcroVax HBcAg with SUMO-Affimer inserted at N-terminus (T=3 VLP)
Method: EM (single particle) / Resolution: 3.5 Å

50833

9fwf

EMDB-50833, PDB-9fwf:
N-VelcroVax HBcAg with SUMO-Affimer inserted at N-terminus (T=4 VLP)
Method: EM (single particle) / Resolution: 3.3 Å

EMDB-50834: N-VelcroVax HBcAg in complex with SUMO-gp1 (T=3 VLP)
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-50835: N-VelcroVax HBcAg in complex with SUMO-gp1 (T=4 VLP)
Method: EM (single particle) / Resolution: 3.5 Å

Source
  • synthetic construct (others)
KeywordsVIRUS LIKE PARTICLE / velcrovax / hepatitis b virus / hepatitis b core antigen / affimer / vaccine / recombinant / vlp / antigen display

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