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TitleCryo-EM structure and evolutionary history of the conjugation surface exclusion protein TraT.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 659, Year 2025
Publish dateJan 14, 2025
AuthorsChloe Seddon / Sophia David / Joshua L C Wong / Naito Ishimoto / Shan He / Jonathan Bradshaw / Wen Wen Low / Gad Frankel / Konstantinos Beis /
PubMed AbstractConjugation plays a major role in dissemination of antimicrobial resistance genes. Following transfer of IncF-like plasmids, recipients become refractory to a second wave of conjugation with the same ...Conjugation plays a major role in dissemination of antimicrobial resistance genes. Following transfer of IncF-like plasmids, recipients become refractory to a second wave of conjugation with the same plasmid via entry (TraS) and surface (TraT) exclusion mechanisms. Here, we show that TraT from the pKpQIL and F plasmids (TraT and TraT) exhibits plasmid surface exclusion specificity. The cryo-EM structures of TraT and TraT reveal that they oligomerise into decameric champagne bottle cork-like structures, which are anchored to the outer membrane via a diacylglycerol and palmitic acid modified α-helical barrel domain. Unexpectedly, we identify chromosomal TraT homologues from multiple Gram-negative phyla which form numerous divergent lineages in a phylogenetic tree of TraT sequences. Plasmid-associated TraT sequences are found in multiple distinct lineages, including two separate clades incorporating TraT from Enterobacteriaceae IncF/F-like and Legionellaceae F-like plasmids. These findings suggest that different plasmid backbones have acquired and co-opted TraT on independent occasions.
External linksNat Commun / PubMed:39809778 / PubMed Central
MethodsEM (single particle)
Resolution2.47 - 2.66 Å
Structure data

50723

9fs5

EMDB-50723, PDB-9fs5:
Cryo-EM structure of the decameric TraT surface exclusion lipoprotein from Escherichia coli (F plasmid)
Method: EM (single particle) / Resolution: 2.66 Å

50728

9fsm

EMDB-50728, PDB-9fsm:
Cryo-EM structure of the decameric TraT surface exclusion lipoprotein from Klebsiella pneumoniae (pKpQIL plasmid)
Method: EM (single particle) / Resolution: 2.47 Å

Chemicals

ChemComp-DGA:
DIACYL GLYCEROL

Source
  • Escherichia (bacteria)
  • escherichia coli (E. coli)
  • klebsiella pneumoniae (bacteria)
KeywordsMEMBRANE PROTEIN / surface exclusion / decamer / diacylglycerol (DAG) modification

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