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TitleNAD-dependent dehydrogenases enable efficient growth of Paracoccus denitrificans on the PET monomer ethylene glycol.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 5845, Year 2025
Publish dateJul 1, 2025
AuthorsMinrui Ren / Danni Li / Holly Addison / Willem E M Noteborn / Elisabeth H Andeweg / Timo Glatter / Johannes H de Winde / Johannes G Rebelein / Meindert H Lamers / Lennart Schada von Borzyskowski /
PubMed AbstractEthylene glycol is a monomer of the plastic polyethylene terephthalate (PET) and an environmental pollutant of increasing concern. Although it is generally accepted that bacteria use ethylene glycol ...Ethylene glycol is a monomer of the plastic polyethylene terephthalate (PET) and an environmental pollutant of increasing concern. Although it is generally accepted that bacteria use ethylene glycol as growth substrate, not all involved enzymes are well understood. Here, we show that Paracoccus denitrificans assimilates ethylene glycol solely via NAD-dependent alcohol and aldehyde dehydrogenases. Using comparative proteomics, we identify a gene cluster that is strongly expressed in the presence of ethylene glycol. We report the functional and structural characterization of EtgB and EtgA, key enzymes encoded by this etg gene cluster. We furthermore show that the transcriptional activator EtgR controls expression of the gene cluster. Adaptive laboratory evolution on ethylene glycol results in faster growth, enabled by increased production of EtgB and EtgA. Bioinformatic analysis reveals that the etg gene cluster is widely distributed among bacteria, suggesting a common role of NAD-dependent dehydrogenases in microbial ethylene glycol assimilation.
External linksNat Commun / PubMed:40592898 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.1 Å
Structure data

50545

9flz

EMDB-50545, PDB-9flz:
Alcohol dehydrogenase
Method: EM (single particle) / Resolution: 3.0 Å

50550

9fm9

EMDB-50550, PDB-9fm9:
Aldehyde dehydrogenase
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-K:
Unknown entry

Source
  • paracoccus denitrificans (bacteria)
KeywordsOXIDOREDUCTASE / Alcohol dehydrogenase / tetramer / Aldehyde dehydrogenase

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