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Structure paper

TitleMolecular basis for the phosphorylation of bacterial tyrosine kinase Wzc.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 3437, Year 2025
Publish dateApr 11, 2025
AuthorsYun Yang / Mariana Batista / Bradley R Clarke / Michelle R Agyare-Tabbi / Haigang Song / Noah M Kuehfuss / Audrey Le Bas / Carol V Robinson / Chris Whitfield / Phillip J Stansfeld / James H Naismith / Jiwei Liu /
PubMed AbstractThe regulation of polymerisation and translocation of biomolecules is fundamental. Wzc, an integral cytoplasmic membrane tyrosine autokinase protein serves as the master regulator of the biosynthesis ...The regulation of polymerisation and translocation of biomolecules is fundamental. Wzc, an integral cytoplasmic membrane tyrosine autokinase protein serves as the master regulator of the biosynthesis and export of many bacterial capsular polysaccharides and exopolysaccharides. Such polysaccharides play essential roles in infection, defence, and some are important industrial products. Wzc comprises a large periplasmic domain, two transmembrane helices and a C-terminal cytoplasmic kinase domain with a tyrosine-rich tail. Wzc regulates polymerisation functions through cycling the formation and dissociation of an octameric complex, driven by changes in the phosphorylation status of the tyrosine-rich tail. E. coli Wzc serves a model for a wider family of polysaccharide co-polymerases. Here, we determine structures of intermediate states with different extents of phosphorylation. Structural and computational data reveal the pre-ordering of the tyrosine-rich tail, the molecular basis underlying the unidirectionality of phosphorylation events, and the underlying structural dynamics on how phosphorylation status is transmitted.
External linksNat Commun / PubMed:40210632 / PubMed Central
MethodsEM (single particle)
Resolution2.49 - 3.0 Å
Structure data

50042

9exo

EMDB-50042, PDB-9exo:
Wzc-K540M-2YE MgADP C1
Method: EM (single particle) / Resolution: 3.0 Å

50043

9exp

EMDB-50043, PDB-9exp:
Wzc-K540M-2YE MgADP C8
Method: EM (single particle) / Resolution: 2.85 Å

50044

9i2q

EMDB-50044, PDB-9i2q:
Wzc-K540M-3YE MgADP C1
Method: EM (single particle) / Resolution: 2.9 Å

50045

9i2r

EMDB-50045, PDB-9i2r:
Wzc-K540M-3YE MgADP C8
Method: EM (single particle) / Resolution: 2.59 Å

50046

9exq

EMDB-50046, PDB-9exq:
Wzc-K540M-3YE-N711Y MgADP C1
Method: EM (single particle) / Resolution: 2.71 Å

50047

9exr

EMDB-50047, PDB-9exr:
Wzc-K540M-3YE-N711Y MgADP C8
Method: EM (single particle) / Resolution: 2.49 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • escherichia coli (E. coli)
KeywordsMEMBRANE PROTEIN / Wzc / Wzc-K540M-2YE / Wzc-K540M-3YE-N711Y / Wzc-K540M-3YE

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