EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Diverse RNA Structures Induce PRC2 Dimerization and Inhibit Histone Methyltransferase Activity.
ジャーナル・号・ページ	bioRxiv, Year 2024
掲載日	2024年8月29日
著者	Jiarui Song / Liqi Yao / Anne R Gooding / Valentin Thron / Vignesh Kasinath / Thomas R Cech
PubMed 要旨	Methyltransferase PRC2 (Polycomb Repressive Complex 2) introduces histone H3K27 trimethylation, a repressive chromatin mark, to tune the differential expression of genes. PRC2 is precisely regulated ...Methyltransferase PRC2 (Polycomb Repressive Complex 2) introduces histone H3K27 trimethylation, a repressive chromatin mark, to tune the differential expression of genes. PRC2 is precisely regulated by accessory proteins, histone post-translational modifications and, notably, RNA. Research on PRC2-associated RNA has mostly focused on the tight-binding G-quadruplex (G4) RNAs, which inhibit PRC2 enzymatic activity in vitro and in cells. Our recent cryo-EM structure provided a molecular mechanism for G4 RNA inactivating PRC2 via dimerization, but it remained unclear how diverse RNAs associate with and regulate PRC2. Here, we show that a single-stranded G-rich RNA and an atypical G4 structure called pUG-fold unexpectedly also mediate near-identical PRC2 dimerization resulting in inhibition of PRC2 methyltransferase activity. The conformational flexibility of arginine-rich loops within subunits EZH2 and AEBP2 of PRC2 can accommodate diverse RNA secondary structures, resulting in protein-RNA and protein-protein interfaces similar to those observed previously with G4 RNA. Furthermore, we address a recent report that failed to detect PRC2-associated RNAs in living cells by demonstrating the insensitivity of PRC2-RNA interaction to photochemical crosslinking. Our results support the significance of RNA-mediated PRC2 regulation by showing that this interaction is not limited to a single RNA secondary structure, consistent with the broad PRC2 transcriptome containing many G-tract RNAs incapable of folding into G4 structures.
リンク	bioRxiv / PubMed:39257770 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 3.4 Å
構造データ	EMDB-46722: Body 1 from multibody refinement of the single-stranded TERRAmut RNA-bound PRC2 dimer 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-46726: Body 2 from multibody refinement of the single-stranded TERRAmut RNA-bound PRC2 dimer 手法: EM (単粒子) / 解像度: 3.4 Å 46751 9dch EMDB-46751, PDB-9dch: Single-stranded RNA-mediated PRC2 dimer 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト)
キーワード	GENE REGULATION / PRC2 / RNA / RNP complex / chromatin modifier