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TitleStructural basis of undecaprenyl phosphate glycosylation leading to polymyxin resistance in Gram-negative bacteria.
Journal, issue, pagesbioRxiv, Year 2025
Publish dateJan 30, 2025
AuthorsKhuram U Ashraf / Mariana Bunoro-Batista / T Bertie Ansell / Ankita Punetha / Stephannie Rosario-Garrido / Emre Firlar / Jason T Kaelber / Phillip J Stansfeld / Vasileios I Petrou /
PubMed AbstractIn Gram-negative bacteria, the enzymatic modification of Lipid A with aminoarabinose (L-Ara4N) leads to resistance against polymyxin antibiotics and cationic antimicrobial peptides. ArnC, an integral ...In Gram-negative bacteria, the enzymatic modification of Lipid A with aminoarabinose (L-Ara4N) leads to resistance against polymyxin antibiotics and cationic antimicrobial peptides. ArnC, an integral membrane glycosyltransferase, attaches a formylated form of aminoarabinose to the lipid undecaprenyl phosphate, enabling its association with the bacterial inner membrane. Here, we present cryo-electron microscopy structures of ArnC from in and nucleotide-bound conformations. These structures reveal a conformational transition that takes place upon binding of the partial donor substrate. Using coarse-grained and atomistic simulations, we provide insights into substrate coordination before and during catalysis, and we propose a catalytic mechanism that may operate on all similar metal-dependent polyprenyl phosphate glycosyltransferases. The reported structures provide a new target for drug design aiming to combat polymyxin resistance.
External linksbioRxiv / PubMed:39974898 / PubMed Central
MethodsEM (single particle)
Resolution2.74 - 2.96 Å
Structure data

43617

8vxh

EMDB-43617, PDB-8vxh:
Cryo-EM Structure of the Glycosyltransferase ArnC from Salmonella enterica in the Apo State Determined on Talos Arctica microscope
Method: EM (single particle) / Resolution: 2.79 Å

43812

9asc

EMDB-43812, PDB-9asc:
Cryo-EM Structure of the Glycosyltransferase ArnC from Salmonella enterica in the UDP-bound State Determined on Talos Arctica microscope
Method: EM (single particle) / Resolution: 2.96 Å

44302

9b77

EMDB-44302, PDB-9b77:
Cryo-EM Structure of the Glycosyltransferase ArnC from Salmonella enterica in the Apo State Determined on Krios microscope
Method: EM (single particle) / Resolution: 2.74 Å

Chemicals

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM

ChemComp-MN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • salmonella enterica subsp. enterica serovar typhimurium str. lt2 (bacteria)
KeywordsTRANSFERASE / Glycosyltransferase / undecaprenyl phosphate / aminoarabinose / polymyxin resistance / GT-A

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