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TitleStructural Insights into the Dynamic Assembly of a YFV sNS1 Tetramer.
Journal, issue, pagesViruses, Vol. 16, Issue 8, Year 2024
Publish dateJul 29, 2024
AuthorsQi Pan / Qiang Chen / Wanqin Zhang / Haizhan Jiao / Lei Yu / Hongli Hu /
PubMed AbstractYellow fever virus (YFV) infections can cause severe diseases in humans, resulting in mass casualties in Africa and the Americas each year. Secretory NS1 (sNS1) is thought to be used as a diagnostic ...Yellow fever virus (YFV) infections can cause severe diseases in humans, resulting in mass casualties in Africa and the Americas each year. Secretory NS1 (sNS1) is thought to be used as a diagnostic marker of flavivirus infections, playing an essential role in the flavivirus life cycle, but little is known about the composition and structure of YFV sNS1. Here, we present that the recombinant YFV sNS1 exists in a heterogeneous mixture of oligomerizations, predominantly in the tetrameric form. The cryoEM structures show that the YFV tetramer of sNS1 is stacked by the hydrophobic interaction between β-roll domains and greasy fingers. According to the 3D variability analysis, the tetramer is in a semi-stable state that may contain multiple conformations with dynamic changes. We believe that our study provides critical insights into the oligomerization of NS1 and will aid the development of NS1-based diagnoses and therapies.
External linksViruses / PubMed:39205186 / PubMed Central
MethodsEM (single particle)
Resolution3.31 - 3.57 Å
Structure data

39898

8zb9

EMDB-39898, PDB-8zb9:
CryoEM structure of non-structural protein 1 dimer from Yellow Fever Virus
Method: EM (single particle) / Resolution: 3.31 Å

39899

8zba

EMDB-39899, PDB-8zba:
CryoEM structure of non-structural protein 1 tetramer from Yellow Fever Virus
Method: EM (single particle) / Resolution: 3.57 Å

Source
  • yellow fever virus 17d
KeywordsVIRAL PROTEIN / flavivirus / non-structural protein 1

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