Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM structures of ryanodine receptors and diamide insecticides reveal the mechanisms of selectivity and resistance.
Journal, issue, pages	Nat Commun, Vol. 15, Issue 1, Page 9056, Year 2024
Publish date	Oct 20, 2024
Authors	Lianyun Lin / Changshi Wang / Wenlan Wang / Heng Jiang / Takashi Murayama / Takuya Kobayashi / Hadiatullah Hadiatullah / Yu Seby Chen / Shunfan Wu / Yiwen Wang / Henryk Korza / Yucheng Gu / Yan Zhang / Jiamu Du / Filip Van Petegem / Zhiguang Yuchi /
PubMed Abstract	The resistance of pests to common insecticides is a global issue that threatens food production worldwide. Diamide insecticides target insect ryanodine receptors (RyRs), causing uncontrolled calcium ...The resistance of pests to common insecticides is a global issue that threatens food production worldwide. Diamide insecticides target insect ryanodine receptors (RyRs), causing uncontrolled calcium release from the sarcoplasmic and endoplasmic reticulum. Despite their high potency and species selectivity, several resistance mutations have emerged. Using a chimeric RyR (chiRyR) approach and cryo-electron microscopy (cryo-EM), we investigate how insect RyRs engage two different diamide insecticides from separate families: flubendiamide, a phthalic acid derivative, and tetraniliprole, an anthranilic compound. Both compounds target the same site in the transmembrane region of the RyR, albeit with different poses, and promote channel opening through coupling with the pore-forming domain. To explore the resistance mechanisms, we also solve two cryo-EM structures of chiRyR carrying the two most common resistance mutations, I4790M and G4946E, both alone and in complex with the diamide insecticide chlorantraniliprole. The resistance mutations perturb the local structure, directly reducing the binding affinity and altering the binding pose. Our findings elucidate the mode of action of different diamide insecticides, reveal the molecular mechanism of resistance mutations, and provide important clues for the development of novel pesticides that can bypass the resistance mutations.
External links	Nat Commun / PubMed:39428398 / PubMed Central
Methods	EM (single particle)
Resolution	3.28 - 3.91 Å
Structure data	38398 8xji EMDB-38398, PDB-8xji: Structure of chimeric RyR complex with flubendiamide Method: EM (single particle) / Resolution: 3.91 Å 38417 8xkh EMDB-38417, PDB-8xkh: Structure of chimeric RyR Complex with tetraniliprole Method: EM (single particle) / Resolution: 3.87 Å 38447 8xlf EMDB-38447, PDB-8xlf: Structure of chimeric RyR Method: EM (single particle) / Resolution: 3.62 Å 38448 8xlh EMDB-38448, PDB-8xlh: Structure of chimeric RyR-I4657M/G4819E Method: EM (single particle) / Resolution: 3.62 Å EMDB-38551: The map of chimeric RyR transmembrane domain in complex with flubendiamide after TMD local refinement Method: EM (single particle) / Resolution: 3.82 Å EMDB-38553: The map of chimeric RyR transmembrane domain in complex with tetraniliprole with TMD local refinement Method: EM (single particle) / Resolution: 3.77 Å 38908 8y40 EMDB-38908, PDB-8y40: Structure of chimeric RyR-I4657M/G4819E complex with chlorantraniliprole Method: EM (single particle) / Resolution: 3.58 Å EMDB-60899: Structure of a chimeric RyR-I4657M/G4819E (local refinement of TMD) Method: EM (single particle) / Resolution: 3.28 Å EMDB-60900: Cryo-EM structure of ref-chiRyR (local refinement of TMD) Method: EM (single particle) / Resolution: 3.68 Å EMDB-60901: cryo-EM structure of chiRyR-I4657M/G4819E complex with CHL (local refinement of TMD) Method: EM (single particle) / Resolution: 3.37 Å
Chemicals	PDB-1lvx: Unknown entry ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM ChemComp-CFF: CAFFEINE / medicationYM PDB-1lv1: Crystal Structure Analysis of the non-active site mutant of tethered HIV-1 protease to 2.1A resolution ChemComp-F0U: 5-bromanyl-N-[4-chloranyl-2-methyl-6-(methylcarbamoyl)phenyl]-2-(3-chloranylpyridin-2-yl)pyrazole-3-carboxamide
Source	oryctolagus cuniculus (rabbit) homo sapiens (human)
Keywords	MEMBRANE PROTEIN / Ryanodine receptor / Ion channel / tetraniliprole / chlorantraniliprole