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TitleStructural and spectroscopic insights into fucoxanthin chlorophyll a/c-binding proteins of diatoms in diverse oligomeric states.
Journal, issue, pagesPlant Commun, Page 101041, Year 2024
Publish dateJul 17, 2024
AuthorsCuicui Zhou / Yue Feng / Zhenhua Li / Lili Shen / Xiaoyi Li / Yumei Wang / Guangye Han / Tingyun Kuang / Cheng Liu / Jian-Ren Shen / Wenda Wang /
PubMed AbstractDiatoms, a group of prevalent marine algae, significantly contribute to global primary productivity. Their substantial biomass is linked to enhanced absorption of blue-green light underwater, ...Diatoms, a group of prevalent marine algae, significantly contribute to global primary productivity. Their substantial biomass is linked to enhanced absorption of blue-green light underwater, facilitated by fucoxanthin chlorophyll a/c-binding proteins (FCPs), exhibiting oligomeric diversity across diatom species. Utilizing mild CN-PAGE analysis on solubilized thylakoid membranes, we displayed monomeric, dimeric, trimeric, tetrameric and pentameric FCPs in diatoms. Mass spectrometry analysis revealed each oligomeric FCP has specific protein compositions, constituting a large Lhcf family of FCP antennas. In addition, we resolved the structures of Thalassiosira pseudonana FCP (Tp-FCP) homotrimer and Chaetoceros gracilis FCP (Cg-FCP) pentamer by cryo-electron microscopy at 2.73 Å and 2.65 Å resolutions, respectively. The distinct pigment composition and organization in various oligomeric FCPs change their blue-green light-harvesting, excitation energy transfer pathways. In comparison to dimeric and trimeric FCPs, Cg-FCP tetramer and Cg-FCP pentamer exhibit stronger absorption by Chls c, red-shifted and broader Chl a fluorescence emission, as well as more robust circular dichroism signals originating from Chl a-carotenoid dimers. These spectroscopic characteristics indicate that Chl a molecules in Cg-FCP tetramer and Cg-FCP pentamer are more heterogeneous than in both dimers and Tp-FCP trimer. The structural and spectroscopic insights provided by this study contribute to a better understanding of the mechanisms that empower diatoms to adapt to fluctuating light environments.
External linksPlant Commun / PubMed:39030906
MethodsEM (single particle)
Resolution2.65 - 2.73 Å
Structure data

36466

8jp3

EMDB-36466, PDB-8jp3:
FCP trimer in diatom Thalassiosira pseudonana
Method: EM (single particle) / Resolution: 2.73 Å

37441

8wck

EMDB-37441, PDB-8wck:
FCP tetramer in Chaetoceros gracilis
Method: EM (single particle) / Resolution: 2.71 Å

37442

8wcl

EMDB-37442, PDB-8wcl:
FCP pentamer in Chaetoceros gracilis
Method: EM (single particle) / Resolution: 2.65 Å

Chemicals

ChemComp-A86:
(3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5,5',6,6',7,8-hexahydro-5,6-epoxy-beta,beta-caroten-3'-

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-KC2:
Chlorophyll c2

ChemComp-KC1:
Chlorophyll c1

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

Source
  • thalassiosira pseudonana ccmp1335 (Diatom)
  • chaetoceros neogracilis (Diatom)
KeywordsPHOTOSYNTHESIS / FCP trimer / FCP tetramers / FCP pentamer in Chaetoceros gracilis

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