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Structure paper

TitlepH-dependent reaction triggering in PmHMGR crystals for time-resolved crystallography.
Journal, issue, pagesBiophys. J., Vol. 123, Page 622-637, Year 2024
Publish dateMar 6, 2023 (structure data deposition date)
AuthorsPurohit, V. / Steussy, C.N. / Rosales, A.R. / Critchelow, C.J. / Schmidt, T. / Helquist, P. / Wiest, O. / Mesecar, A. / Cohen, A.E. / Stauffacher, C.V.
External linksBiophys. J. / PubMed:38327055
MethodsX-ray diffraction
Resolution1.65 - 2.07 Å
Structure data

PDB-8gdn:
Structure of PmHMGR bound to mevalonate, CoA and NAD.
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

PDB-8sz6:
PmHMGR bound to mevaldehyde and CoA
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-8vlq:
Structure of PmHMGR bound to mevalonate, CoA and NAD 5 minutes after reaction initiation at pH 9
Method: X-RAY DIFFRACTION / Resolution: 2.07 Å

Chemicals

ChemComp-COA:
COENZYME A

ChemComp-MEV:
(R)-MEVALONATE

ChemComp-SO4:
SULFATE ION

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-HOH:
WATER


ChemComp, No image

ChemComp-ZKE:
Unknown entry


ChemComp, No image

ChemComp-XII:
Unknown entry

PDB-1afy:
Unknown entry

ChemComp-NAI:
1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

Source
  • pseudomonas sp. 'mevalonii' (bacteria)
KeywordsSTRUCTURAL PROTEIN / Non-Rossmann fold / Homodimer / Reductase / HMG-CoA / OXIDOREDUCTASE

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