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TitleStructural basis for potent neutralization of human respirovirus type 3 by protective single-domain camelid antibodies.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 5458, Year 2024
Publish dateJun 27, 2024
AuthorsNicole V Johnson / Revina C van Scherpenzeel / Mark J G Bakkers / Ajit R Ramamohan / Daan van Overveld / Lam Le / Johannes P M Langedijk / Joost A Kolkman / Jason S McLellan /
PubMed AbstractRespirovirus 3 is a leading cause of severe acute respiratory infections in vulnerable human populations. Entry into host cells is facilitated by the attachment glycoprotein and the fusion ...Respirovirus 3 is a leading cause of severe acute respiratory infections in vulnerable human populations. Entry into host cells is facilitated by the attachment glycoprotein and the fusion glycoprotein (F). Because of its crucial role, F represents an attractive therapeutic target. Here, we identify 13 F-directed heavy-chain-only antibody fragments that neutralize recombinant respirovirus 3. High-resolution cryo-EM structures of antibody fragments bound to the prefusion conformation of F reveal three distinct, previously uncharacterized epitopes. All three antibody fragments bind quaternary epitopes on F, suggesting mechanisms for neutralization that may include stabilization of the prefusion conformation. Studies in cotton rats demonstrate the prophylactic efficacy of these antibody fragments in reducing viral load in the lungs and nasal passages. These data highlight the potential of heavy-chain-only antibody fragments as effective interventions against respirovirus 3 infection and identify neutralizing epitopes that can be targeted for therapeutic development.
External linksNat Commun / PubMed:38937429 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.4 Å
Structure data

42983

8v5k

EMDB-42983, PDB-8v5k:
Structure of the Human Respirovirus 3 Fusion Protein Bound to Camelid Nanobodies 4C03 and 4C06
Method: EM (single particle) / Resolution: 2.6 Å

42987

8v62

EMDB-42987, PDB-8v62:
Structure of the Human Respirovirus 3 Fusion Protein Bound to Camelid Nanobodies 1D10 and 4C06
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • human respirovirus 3
  • lama glama (llama)
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / viral fusion protein / camelid nanobodies / viral glycoprotein / membrane fusion / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex

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