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TitleMolecular mechanisms linking missense ACTG2 mutations to visceral myopathy.
Journal, issue, pagesSci Adv, Vol. 10, Issue 22, Page eadn6615, Year 2024
Publish dateMay 31, 2024
AuthorsRachel H Ceron / Faviolla A Báez-Cruz / Nicholas J Palmer / Peter J Carman / Malgorzata Boczkowska / Robert O Heuckeroth / E Michael Ostap / Roberto Dominguez /
PubMed AbstractVisceral myopathy is a life-threatening disease characterized by muscle weakness in the bowel, bladder, and uterus. Mutations in smooth muscle γ-actin (ACTG2) are the most common cause of the ...Visceral myopathy is a life-threatening disease characterized by muscle weakness in the bowel, bladder, and uterus. Mutations in smooth muscle γ-actin (ACTG2) are the most common cause of the disease, but the mechanisms by which the mutations alter muscle function are unknown. Here, we examined four prevalent ACTG2 mutations (R40C, R148C, R178C, and R257C) that cause different disease severity and are spread throughout the actin fold. R178C displayed premature degradation, R148C disrupted interactions with actin-binding proteins, R40C inhibited polymerization, and R257C destabilized filaments. Because these mutations are heterozygous, we also analyzed 50/50 mixtures with wild-type (WT) ACTG2. The WT/R40C mixture impaired filament nucleation by leiomodin 1, and WT/R257C produced filaments that were easily fragmented by smooth muscle myosin. Smooth muscle tropomyosin isoform Tpm1.4 partially rescued the defects of R40C and R257C. Cryo-electron microscopy structures of filaments formed by R40C and R257C revealed disrupted intersubunit contacts. The biochemical and structural properties of the mutants correlate with their genotype-specific disease severity.
External linksSci Adv / PubMed:38820162 / PubMed Central
MethodsEM (helical sym.)
Resolution2.45 - 2.54 Å
Structure data

42918

8v2o

EMDB-42918, PDB-8v2o:
Cryo-EM Structure of Wildtype Smooth Muscle Gamma Actin (ACTG2)
Method: EM (helical sym.) / Resolution: 2.45 Å

42939

8v30

EMDB-42939, PDB-8v30:
Smooth Muscle Gamma Actin (ACTG2) Filament Mutant R40C
Method: EM (helical sym.) / Resolution: 2.54 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsSTRUCTURAL PROTEIN / Filament / Actin / Smooth Muscle / CYTOSOLIC PROTEIN

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