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TitleThe NCR1 transporter is an antimalarial target that exports cholesterol from the parasite's plasma membrane.
Journal, issue, pagesSci Adv, Vol. 10, Issue 51, Page eadq6651, Year 2024
Publish dateDec 20, 2024
AuthorsZhemin Zhang / Meinan Lyu / Xu Han / Sepalika Bandara / Meng Cui / Eva S Istvan / Xinran Geng / Marios L Tringides / William D Gregor / Masaru Miyagi / Jenna Oberstaller / John H Adams / Youwei Zhang / Marvin T Nieman / Johannes von Lintig / Daniel E Goldberg / Edward W Yu /
PubMed AbstractMalaria, a devastating parasitic infection, is the leading cause of death in many developing countries. Unfortunately, the most deadliest causative agent of malaria, , has developed resistance to ...Malaria, a devastating parasitic infection, is the leading cause of death in many developing countries. Unfortunately, the most deadliest causative agent of malaria, , has developed resistance to nearly all currently available antimalarial drugs. The Niemann-Pick type C1-related (PfNCR1) transporter has been identified as a druggable target, but its structure and detailed molecular mechanism are not yet available. Here, we present three structures of PfNCR1 with and without the functional inhibitor MMV009108 at resolutions between 2.98 and 3.81 Å using single-particle cryo-electron microscopy (cryo-EM), suggesting that PfNCR1 binds cholesterol and forms a cholesterol transport tunnel to modulate the composition of the parasite plasma membrane. Cholesterol efflux assays show that PfNCR1 is an exporter capable of extruding cholesterol from the membrane. Additionally, the inhibition mechanism of MMV009108 appears to be due to a direct blockage of PfNCR1, preventing this transporter from shuttling cholesterol.
External linksSci Adv / PubMed:39693420 / PubMed Central
MethodsEM (single particle)
Resolution2.98 - 3.81 Å
Structure data

42854

8v0g

EMDB-42854, PDB-8v0g:
plasmodium falciparum Niemann-Pick type C1-related protein form I
Method: EM (single particle) / Resolution: 3.11 Å

42878

8v12

EMDB-42878, PDB-8v12:
plasmodium falciparum Niemann-Pick type C1-related protein form II
Method: EM (single particle) / Resolution: 3.81 Å

42885

8v1g

EMDB-42885, PDB-8v1g:
plasmodium falciparum Niemann-Pick type C1-related protein bound with MMV009108
Method: EM (single particle) / Resolution: 2.98 Å

Chemicals

ChemComp-CLR:
CHOLESTEROL

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose


ChemComp, No image

ChemComp-Y6F:
Unknown entry

Source
  • plasmodium falciparum 3d7 (eukaryote)
KeywordsMEMBRANE PROTEIN / Niemann-Pick type C1-related protein / NCR1 / cholesterol transporter / plasmodium falciparum

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