EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	P-type ATPase magnesium transporter MgtA acts as a dimer.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 32, Issue 9, Page 1633-1643, Year 2025
掲載日	2025年6月23日
著者	Rilee Zeinert / Fei Zhou / Pedro Franco / Jonathan Zöller / Zaid K Madni / Henry Lessen / L Aravind / Julian D Langer / Alexander J Sodt / Gisela Storz / Doreen Matthies /
PubMed 要旨	Magnesium (Mg) uptake systems are present in all domains of life, consistent with the vital role of this ion. P-type ATPase Mg importers are required for bacterial growth when Mg is limiting or ...Magnesium (Mg) uptake systems are present in all domains of life, consistent with the vital role of this ion. P-type ATPase Mg importers are required for bacterial growth when Mg is limiting or during pathogenesis. However, insights into their mechanisms of action are missing. Here we solved the cryo-EM structure of the Mg transporter MgtA from Escherichia coli. We obtained high-resolution structures of both homodimeric (2.9 Å) and monomeric (3.6 Å) forms. The dimer structure is formed by multiple contacts between residues in adjacent soluble N and P subdomains. Our structures revealed an ion, assigned as Mg, in the transmembrane segment. Moreover, we detected two cytoplasmic ion-binding sites and determined the structure of the N-terminal tail. Sequence conservation, mutagenesis and ATPase assays indicate dimerization, the ion-binding sites and the N-terminal tail facilitate cation transport or serve regulatory roles.
リンク	Nat Struct Mol Biol / PubMed:40550995 / PubMed Central
手法	EM (単粒子)
解像度	2.93 - 3.87 Å
構造データ	42794 8uy7 EMDB-42794, PDB-8uy7: Magnesium transporter MgtA dimer from E. coli in 5 mM MgCl2 手法: EM (単粒子) / 解像度: 2.93 Å 42795 8uy8 EMDB-42795, PDB-8uy8: Magnesium transporter MgtA dimer from E. coli in 5 mM MgCl2 in C1 手法: EM (単粒子) / 解像度: 3.03 Å 42796 8uy9 EMDB-42796, PDB-8uy9: Magnesium transporter MgtA monomer from E. coli in 5 mM MgCl2 手法: EM (単粒子) / 解像度: 3.65 Å 42797 8uya EMDB-42797, PDB-8uya: Magnesium transporter MgtA dimer from E. coli in 5 mM MgCl2 and 5 mM ATP 手法: EM (単粒子) / 解像度: 3.72 Å 42798 8uyb EMDB-42798, PDB-8uyb: Magnesium transporter MgtA dimer from E. coli in 5 mM MgCl2 and 5 mM ATPyS 手法: EM (単粒子) / 解像度: 3.87 Å 42799 8uyc EMDB-42799, PDB-8uyc: Magnesium transporter MgtA dimer from E. coli in 5 mM MgCl2 and 5 mM ADP 手法: EM (単粒子) / 解像度: 3.75 Å
化合物	ChemComp-MG: Unknown entry ChemComp-HOH: WATER ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子YM ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, エネルギー貯蔵分子類似体YM
由来	escherichia coli (大腸菌) escherichia coli k-12 (大腸菌)
キーワード	MEMBRANE PROTEIN / magnesium / transport / dimer / oligomer / cryo-EM / P-type ATPase / ion translocation