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TitleStructure of a Rhs effector clade domain provides mechanistic insights into type VI secretion system toxin delivery.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 8709, Year 2024
Publish dateOct 8, 2024
AuthorsBrooke K Hayes / Marina Harper / Hariprasad Venugopal / Jessica M Lewis / Amy Wright / Han-Chung Lee / Joel R Steele / David L Steer / Ralf B Schittenhelm / John D Boyce / Sheena McGowan /
PubMed AbstractThe type VI secretion system (T6SS) is a molecular machine utilised by many Gram-negative bacteria to deliver antibacterial toxins into adjacent cells. Here we present the structure of Tse15, a T6SS ...The type VI secretion system (T6SS) is a molecular machine utilised by many Gram-negative bacteria to deliver antibacterial toxins into adjacent cells. Here we present the structure of Tse15, a T6SS Rhs effector from the nosocomial pathogen Acinetobacter baumannii. Tse15 forms a triple layered β-cocoon Rhs domain with an N-terminal α-helical clade domain and an unfolded C-terminal toxin domain inside the Rhs cage. Tse15 is cleaved into three domains, through independent auto-cleavage events involving aspartyl protease activity for toxin self-cleavage and a nucleophilic glutamic acid for N-terminal clade cleavage. Proteomic analyses identified that significantly more peptides from the N-terminal clade and toxin domains were secreted than from the Rhs cage, suggesting toxin delivery often occurs without the cage. We propose the clade domain acts as an internal chaperone to mediate toxin tethering to the T6SS machinery. Conservation of the clade domain in other Gram-negative bacteria suggests this may be a common mechanism for delivery.
External linksNat Commun / PubMed:39379370 / PubMed Central
MethodsEM (single particle)
Resolution1.77 - 3.08 Å
Structure data

42775

8uxt

EMDB-42775, PDB-8uxt:
Acinetobacter baumannii Tse15 Rhs effector, toxin cleavage mutant (D1369N, D1391N)
Method: EM (single particle) / Resolution: 1.77 Å

42792

8uy4

EMDB-42792, PDB-8uy4:
Acinetobacter baumannii Tse15 Rhs effector
Method: EM (single particle) / Resolution: 3.08 Å

Source
  • acinetobacter baumannii ab307-0294 (bacteria)
KeywordsTOXIN / Rhs-effector / Rhs cargo effector / Type 6 secretion system effector / Acinetobacter baumannii toxin / YD-repeat protein / T6SS. / Type VI secretion system

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