EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	An RNA modification prevents extended codon-anticodon interactions from facilitating +1 frameshifting.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 7392, Year 2025
掲載日	2025年8月11日
著者	Evelyn M Kimbrough / Ha An Nguyen / Haixing Li / Jacob M Mattingly / Nevette A Bailey / Wei Ning / Howard Gamper / Ya-Ming Hou / Ruben L Gonzalez / Christine M Dunham /
PubMed 要旨	RNA post-transcriptional modifications act by stabilizing the functional conformations of RNA. While their role in messenger RNA (mRNA) decoding is well established, it is less clear how transfer RNA ...RNA post-transcriptional modifications act by stabilizing the functional conformations of RNA. While their role in messenger RNA (mRNA) decoding is well established, it is less clear how transfer RNA (tRNA) modifications outside the anticodon contribute to tRNA stability and accurate protein synthesis. Absence of such modifications causes translation errors, including mRNA frameshifting. By integrating single-molecule fluorescence resonance energy transfer and cryogenic electron microscopy, we demonstrate that the N-methylguanosine (mG) modification at position 37 of Escherichia coli tRNA is necessary and sufficient for modulating the conformational energy of this tRNA on the ribosome so as to suppress +1 frameshifting otherwise induced by this tRNA. Six structures of E. coli ribosomal complexes carrying tRNA lacking mG37 show this tRNA forms four and even five codon-anticodon base pairs as it moves into the +1 frame, allowing direct visualization of the long-standing hypothesis that a four base pair codon-anticodon can form during +1 frameshifting.
リンク	Nat Commun / PubMed:40789848 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 4.0 Å
構造データ	42495 8urm EMDB-42495, PDB-8urm: E. coli 70S ribosome with unmodified tRNAPro(GGG) bound to slippery P-site CCC-C codon and tRNAVal(UAC) in the A site 手法: EM (単粒子) / 解像度: 3.0 Å 42541 8utj EMDB-42541, PDB-8utj: E. coli 70S ribosome with unmodified lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the 0 frame 手法: EM (単粒子) / 解像度: 3.5 Å 42714 8ux8 EMDB-42714, PDB-8ux8: E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) bound to slippery P-site CCC-C codon in the +1 mRNA reading frame 手法: EM (単粒子) / 解像度: 3.6 Å 42721 8uxb EMDB-42721, PDB-8uxb: E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon 手法: EM (単粒子) / 解像度: 2.9 Å 42840 8uzg EMDB-42840, PDB-8uzg: E. coli 70S ribosome with unmodified *e/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon 手法: EM (単粒子) / 解像度: 3.7 Å 42852 8v03 EMDB-42852, PDB-8v03: E. coli 70S ribosome with unmodified P/E-tRNAPro(GGG) bound to slippery P-site CCC-C codon 手法: EM (単粒子) / 解像度**: 4.0 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER ChemComp-LYS: LYSINE / 2-アンモニオ-2-デアミノ-L-リシンアニオン
由来	escherichia coli (大腸菌)
キーワード	RIBOSOME / 70S ribosome / tRNA / mRNA / frameshift