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TitlePhotoreception and signaling in bacterial phytochrome revealed by single-particle cryo-EM.
Journal, issue, pagesSci Adv, Vol. 10, Issue 32, Page eadq0653, Year 2024
Publish dateAug 9, 2024
AuthorsTek Narsingh Malla / Carolina Hernandez / Srinivasan Muniyappan / David Menendez / Dorina Bizhga / Joshua H Mendez / Peter Schwander / Emina A Stojković / Marius Schmidt /
PubMed AbstractPhytochromes are red-light photoreceptors discovered in plants with homologs in bacteria and fungi that regulate a variety of physiological responses. They display a reversible photocycle between two ...Phytochromes are red-light photoreceptors discovered in plants with homologs in bacteria and fungi that regulate a variety of physiological responses. They display a reversible photocycle between two distinct states: a red-light-absorbing Pr state and a far-red light-absorbing Pfr state. The photoconversion regulates the activity of an enzymatic domain, usually a histidine kinase (HK). The molecular mechanism that explains how light controls the HK activity is not understood because structures of unmodified bacterial phytochromes with HK activity are missing. Here, we report three cryo-electron microscopy structures of a wild-type bacterial phytochrome with HK activity determined as Pr and Pfr homodimers and as a Pr/Pfr heterodimer with individual subunits in distinct states. We propose that the Pr/Pfr heterodimer is a physiologically relevant signal transduction intermediate. Our results offer insight into the molecular mechanism that controls the enzymatic activity of the HK as part of a bacterial two-component system that perceives and transduces light signals.
External linksSci Adv / PubMed:39121216 / PubMed Central
MethodsEM (single particle)
Resolution3.75 - 4.61 Å
Structure data

42448

8uph

EMDB-42448: Pr state of Stigmatella aurantiaca bacteriophytochrome 2
PDB-8uph: Prf state of Stigmatella aurantiaca bacteriophytochrome 2
Method: EM (single particle) / Resolution: 4.13 Å

42450

8upk

EMDB-42450, PDB-8upk:
Pr/Pfr heterodimer (hybrid) state of Stigmatella aurantiaca bacteriophytochrome 2
Method: EM (single particle) / Resolution: 3.75 Å

42452

8upm

EMDB-42452, PDB-8upm:
Pfr state of Stigmatella aurantiaca bacteriophytochrome 2
Method: EM (single particle) / Resolution: 3.75 Å

42469

8uqi

EMDB-42469, PDB-8uqi:
Pr/Pfr heterodimeric state of photosensory core module of Stigmatella aurantiaca bacteriophytochrome 2
Method: EM (single particle) / Resolution: 4.5 Å

42472

8uqk

EMDB-42472, PDB-8uqk:
Pfr state of photosensory core module of Stigmatella aurantiaca bacteriophytochrome 2
Method: EM (single particle) / Resolution: 4.61 Å

Chemicals

ChemComp-BLA:
BILIVERDINE IX ALPHA

Source
  • stigmatella aurantiaca (bacteria)
KeywordsSIGNALING PROTEIN / Red light photoreceptor / two component system

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