EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Visualization of the Cdc48 AAA+ ATPase protein unfolding pathway.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 7505, Year 2024
掲載日	2024年8月29日
著者	Ian Cooney / Heidi L Schubert / Karina Cedeno / Olivia N Fisher / Richard Carson / John C Price / Christopher P Hill / Peter S Shen /
PubMed 要旨	The Cdc48 AAA+ ATPase is an abundant and essential enzyme that unfolds substrates in multiple protein quality control pathways. The enzyme includes two conserved AAA+ ATPase motor domains, D1 and D2, ...The Cdc48 AAA+ ATPase is an abundant and essential enzyme that unfolds substrates in multiple protein quality control pathways. The enzyme includes two conserved AAA+ ATPase motor domains, D1 and D2, that assemble as hexameric rings with D1 stacked above D2. Here, we report an ensemble of native structures of Cdc48 affinity purified from budding yeast lysate in complex with the adaptor Shp1 in the act of unfolding substrate. Our analysis reveals a continuum of structural snapshots that spans the entire translocation cycle. These data uncover elements of Shp1-Cdc48 interactions and support a 'hand-over-hand' mechanism in which the sequential movement of individual subunits is closely coordinated. D1 hydrolyzes ATP and disengages from substrate prior to D2, while D2 rebinds ATP and re-engages with substrate prior to D1, thereby explaining the dominant role played by the D2 motor in substrate translocation/unfolding.
リンク	Nat Commun / PubMed:39209885 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 4.3 Å
構造データ	41992 8u7t EMDB-41992, PDB-8u7t: Substrate-bound Cdc48, Class 1 手法: EM (単粒子) / 解像度: 3.3 Å 42025 8u8i EMDB-42025, PDB-8u8i: Cdc48-Shp1 unfolding native substrate, Class 4 手法: EM (単粒子) / 解像度: 3.5 Å 42032 8u9c EMDB-42032, PDB-8u9c: Cdc48-Shp1 unfolding native substrate, Class 5 手法: EM (単粒子) / 解像度: 3.7 Å 42038 8u9p EMDB-42038, PDB-8u9p: Cdc48-Shp1 unfolding native substrate, Class 2 手法: EM (単粒子) / 解像度: 3.2 Å 42039 8u9q EMDB-42039, PDB-8u9q: Cdc48-Shp1 unfolding native substrate, Class 6 手法: EM (単粒子) / 解像度: 4.3 Å 42042 8u9z EMDB-42042, PDB-8u9z: Cdc48-Shp1 unfolding native substrate, Class 7 手法: EM (単粒子) / 解像度: 3.8 Å 42043 8ua0 EMDB-42043, PDB-8ua0: Cdc48-Shp1 unfolding native substrate, Class 8 手法: EM (単粒子) / 解像度: 3.5 Å 42047 8ua1 EMDB-42047, PDB-8ua1: Cdc48-Shp1 unfolding native substrate, Class 9 手法: EM (単粒子) / 解像度: 3.4 Å 42057 8uaa EMDB-42057, PDB-8uaa: Cdc48-Shp1 unfolding native substrate, Class 3 手法: EM (単粒子) / 解像度: 3.4 Å 42076 8ub4 EMDB-42076, PDB-8ub4: Cdc48-Shp1 unfolding native substrate, consensus structure 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-08T: [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM
由来	saccharomyces cerevisiae (パン酵母)
キーワード	CHAPERONE / unfoldase / AAA ATPase / p97 / Cdc48