Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Molecular insights into capsular polysaccharide secretion.
Journal, issue, pages	Nature, Vol. 628, Issue 8009, Page 901-909, Year 2024
Publish date	Apr 3, 2024
Authors	Jeremi Kuklewicz / Jochen Zimmer /
PubMed Abstract	Capsular polysaccharides (CPSs) fortify the cell boundaries of many commensal and pathogenic bacteria. Through the ABC-transporter-dependent biosynthesis pathway, CPSs are synthesized intracellularly ...Capsular polysaccharides (CPSs) fortify the cell boundaries of many commensal and pathogenic bacteria. Through the ABC-transporter-dependent biosynthesis pathway, CPSs are synthesized intracellularly on a lipid anchor and secreted across the cell envelope by the KpsMT ABC transporter associated with the KpsE and KpsD subunits. Here we use structural and functional studies to uncover crucial steps of CPS secretion in Gram-negative bacteria. We show that KpsMT has broad substrate specificity and is sufficient for the translocation of CPSs across the inner bacterial membrane, and we determine the cell surface organization and localization of CPSs using super-resolution fluorescence microscopy. Cryo-electron microscopy analyses of the KpsMT-KpsE complex in six different states reveal a KpsE-encaged ABC transporter, rigid-body conformational rearrangements of KpsMT during ATP hydrolysis and recognition of a glycolipid inside a membrane-exposed electropositive canyon. In vivo CPS secretion assays underscore the functional importance of canyon-lining basic residues. Combined, our analyses suggest a molecular model of CPS secretion by ABC transporters.
External links	Nature / PubMed:38570679 / PubMed Central
Methods	EM (single particle)
Resolution	3.1 - 4.4 Å
Structure data	41592 8tsh EMDB-41592, PDB-8tsh: S. thermodepolymerans KpsMT(E151Q)-KpsE in complex with ATP Method: EM (single particle) / Resolution: 3.1 Å 41593 8tsi EMDB-41593, PDB-8tsi: S. thermodepolymerans KpsMT-KpsE in complex with ADP:AlF4- Method: EM (single particle) / Resolution: 4.4 Å 41595 8tsl EMDB-41595, PDB-8tsl: S. thermodepolymerans KpsM-KpsE in Apo 2 state with rigid body fitted KpsT Method: EM (single particle) / Resolution: 3.4 Å 41601 8tsw EMDB-41601, PDB-8tsw: S. thermodepolymerans KpsMT-KpsE Apo 1 Method: EM (single particle) / Resolution: 3.1 Å 41602 8tt3 EMDB-41602, PDB-8tt3: S. thermodepolymerans KpsM-KpsE in Glycolipid 2 state with rigid body fitted KpsT Method: EM (single particle) / Resolution: 3.4 Å 41626 8tun EMDB-41626, PDB-8tun: S. thermodepolymerans KpsM-KpsE in Glycolipid 1 state with rigid body fitted KpsT Method: EM (single particle) / Resolution: 3.4 Å EMDB-41677: S. thermodepolymerans KpsMT-KpsE Apo 1 - consensus map Method: EM (single particle) / Resolution: 3.1 Å EMDB-41678: S. thermodepolymerans KpsMT-KpsE Apo 1 - KpsT-focused map Method: EM (single particle) / Resolution: 3.3 Å EMDB-41681: S. thermodepolymerans KpsMT(E151Q)-KpsE in complex with ATP - consensus map Method: EM (single particle) / Resolution: 3.1 Å EMDB-41682: S. thermodepolymerans KpsMT(E151Q)-KpsE in complex with ATP - crown focused map Method: EM (single particle) / Resolution: 3.3 Å EMDB-41697: S. thermodepolymerans KpsMT-KpsE in Apo 2 state - consensus map Method: EM (single particle) / Resolution: 3.3 Å EMDB-41698: S. thermodepolymerans KpsMT-KpsE in Apo 2 state - KpsT focused map Method: EM (single particle) / Resolution: 3.5 Å EMDB-41718: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 1 - consensus map Method: EM (single particle) / Resolution: 3.4 Å EMDB-41720: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 1 - KpsT focused map Method: EM (single particle) / Resolution: 3.4 Å EMDB-41721: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 1 - KpsM focused map Method: EM (single particle) / Resolution: 3.4 Å EMDB-41771: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 2 - consensus map Method: EM (single particle) / Resolution: 3.4 Å EMDB-41772: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 2 - KpsT focused map Method: EM (single particle) / Resolution: 3.4 Å EMDB-41773: S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 2 - KpsM focused map Method: EM (single particle) / Resolution: 3.4 Å
Chemicals	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM / Adenosine triphosphate ChemComp-MG: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM / Adenosine diphosphate ChemComp-ALF: TETRAFLUOROALUMINATE ION ChemComp, No image ChemComp-KJ9: Unknown entry
Source	caldimonas thermodepolymerans (bacteria)
Keywords	MEMBRANE PROTEIN / ABC transporter / Capsular polysaccharide